RNA genome stability of Toscana virus during serial transovarial transmission in the sandfly Phlebotomus perniciosus

Pamuk A. Bilsel; Robert B. Tesh; Stuart T. Nichol

doi:10.1016/0168-1702(88)90069-X

RNA genome stability of Toscana virus during serial transovarial transmission in the sandfly Phlebotomus perniciosus

Pamuk A. Bilsel, Robert B. Tesh, Stuart T. Nichol

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We have carried out a T1 ribonuclease fingerprinting analysis of the RNA genomes of Toscana virus isolates from successive generations of an experimentally virus-infected laboratory colony of Phlebotomus perniciosus sandflies. This analysis detected no virus RNA genome changes during transovarial transmission of the virus over 12 sandfly generations (a period of almost 2 years). These results demonstrate that although RNA viruses can exhibit high rates of mutational change under a variety of conditions, Toscana virus RNA genomes can be maintained in a stable manner during repeated transovarial virus transmission in the natural insect host. The implications of these results for insect RNA virus evolution are discussed.

Original language	English (US)
Pages (from-to)	87-94
Number of pages	8
Journal	Virus Research
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/0168-1702(88)90069-X
State	Published - Aug 1988
Externally published	Yes

Keywords

Insect model
Phlebovirus
RNA virus evolution
T1 RNase fingerprinting

ASJC Scopus subject areas

Infectious Diseases
Cancer Research
Virology

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10.1016/0168-1702(88)90069-X

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title = "RNA genome stability of Toscana virus during serial transovarial transmission in the sandfly Phlebotomus perniciosus",

abstract = "We have carried out a T1 ribonuclease fingerprinting analysis of the RNA genomes of Toscana virus isolates from successive generations of an experimentally virus-infected laboratory colony of Phlebotomus perniciosus sandflies. This analysis detected no virus RNA genome changes during transovarial transmission of the virus over 12 sandfly generations (a period of almost 2 years). These results demonstrate that although RNA viruses can exhibit high rates of mutational change under a variety of conditions, Toscana virus RNA genomes can be maintained in a stable manner during repeated transovarial virus transmission in the natural insect host. The implications of these results for insect RNA virus evolution are discussed.",

keywords = "Insect model, Phlebovirus, RNA virus evolution, T1 RNase fingerprinting",

author = "Bilsel, {Pamuk A.} and Tesh, {Robert B.} and Nichol, {Stuart T.}",

note = "Funding Information: We thank Drs. Jonathan Smith and Dan Kolakofsky for advice concerning virus purification and Terri Williams and Sonny Rosenberg for excellent technical assistance. This work was supported by funds from the University of Nevada Reno Foundation and College of Agriculture to S.T.N., and by contract DAMD 17-85-C-5023 from the U.S. Army Medical Research and Development Command to R.B.T.",

year = "1988",

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language = "English (US)",

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journal = "Virus Research",

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AU - Bilsel, Pamuk A.

AU - Tesh, Robert B.

AU - Nichol, Stuart T.

N1 - Funding Information: We thank Drs. Jonathan Smith and Dan Kolakofsky for advice concerning virus purification and Terri Williams and Sonny Rosenberg for excellent technical assistance. This work was supported by funds from the University of Nevada Reno Foundation and College of Agriculture to S.T.N., and by contract DAMD 17-85-C-5023 from the U.S. Army Medical Research and Development Command to R.B.T.

PY - 1988/8

Y1 - 1988/8

N2 - We have carried out a T1 ribonuclease fingerprinting analysis of the RNA genomes of Toscana virus isolates from successive generations of an experimentally virus-infected laboratory colony of Phlebotomus perniciosus sandflies. This analysis detected no virus RNA genome changes during transovarial transmission of the virus over 12 sandfly generations (a period of almost 2 years). These results demonstrate that although RNA viruses can exhibit high rates of mutational change under a variety of conditions, Toscana virus RNA genomes can be maintained in a stable manner during repeated transovarial virus transmission in the natural insect host. The implications of these results for insect RNA virus evolution are discussed.

AB - We have carried out a T1 ribonuclease fingerprinting analysis of the RNA genomes of Toscana virus isolates from successive generations of an experimentally virus-infected laboratory colony of Phlebotomus perniciosus sandflies. This analysis detected no virus RNA genome changes during transovarial transmission of the virus over 12 sandfly generations (a period of almost 2 years). These results demonstrate that although RNA viruses can exhibit high rates of mutational change under a variety of conditions, Toscana virus RNA genomes can be maintained in a stable manner during repeated transovarial virus transmission in the natural insect host. The implications of these results for insect RNA virus evolution are discussed.

KW - Insect model

KW - Phlebovirus

KW - RNA virus evolution

KW - T1 RNase fingerprinting

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RNA genome stability of Toscana virus during serial transovarial transmission in the sandfly Phlebotomus perniciosus

Abstract

Keywords

ASJC Scopus subject areas

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