Abstract
Machupo virus (MACV) is the etiological agent of Bolivian hemorrhagic fever (BHF), a reemerging and neglected tropical disease associated with high mortality. The prototypical strain of MACV, Carvallo, was isolated from a human patient in 1963, but minimal in vitro and in vivo characterization has been reported. To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned cDNAs. The recombinant MACV (rMACV) had in vitro growth properties similar to those of the parental MACV. Both viruses caused similar disease development in alpha/beta and gamma interferon receptor knockout mice, including neurological disease development and high mortality. In addition, we have identified a novel murine model with mortality and neurological disease similar to BHF disease reported in humans and nonhuman primates.
Original language | English (US) |
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Pages (from-to) | 1914-1923 |
Number of pages | 10 |
Journal | Journal of virology |
Volume | 88 |
Issue number | 4 |
DOIs | |
State | Published - Feb 2014 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology