RepliVAX WN, a single-cycle flavivirus vaccine to prevent West Nile disease, elicits durable protective immunity in hamsters

Douglas G. Widman; Tomohiro Ishikawa; Evandro R. Winkelmann; Ernesto Infante; Nigel Bourne; Peter W. Mason

doi:10.1016/j.vaccine.2009.07.016

RepliVAX WN, a single-cycle flavivirus vaccine to prevent West Nile disease, elicits durable protective immunity in hamsters

Douglas G. Widman, Tomohiro Ishikawa, Evandro R. Winkelmann, Ernesto Infante, Nigel Bourne, Peter W. Mason

Pediatrics

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

West Nile virus (WNV) causes significant disease, yet no vaccines exist to prevent WN disease in humans. We have previously reported that RepliVAX WN is a safe and efficacious vaccine in mouse and hamster models of WN disease. Here, we report that vaccination of hamsters with RepliVAX WN induces antibody responses that remain stable for at least 6 months. Furthermore, animals challenged with virulent WNV 6 months after vaccination were protected from disease as well as those challenged 2 months post-vaccination, with no vaccinated animals succumbing to WNV challenge. These results indicate that RepliVAX is capable of inducing durable protective immunity after a single dose.

Original language	English (US)
Pages (from-to)	5550-5553
Number of pages	4
Journal	Vaccine
Volume	27
Issue number	41
DOIs	https://doi.org/10.1016/j.vaccine.2009.07.016
State	Published - Sep 18 2009

Keywords

Antibody
Hamster
Live-attenuated vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2009.07.016

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@article{28414c797a7f437fbebee3b38eba0557,

title = "RepliVAX WN, a single-cycle flavivirus vaccine to prevent West Nile disease, elicits durable protective immunity in hamsters",

abstract = "West Nile virus (WNV) causes significant disease, yet no vaccines exist to prevent WN disease in humans. We have previously reported that RepliVAX WN is a safe and efficacious vaccine in mouse and hamster models of WN disease. Here, we report that vaccination of hamsters with RepliVAX WN induces antibody responses that remain stable for at least 6 months. Furthermore, animals challenged with virulent WNV 6 months after vaccination were protected from disease as well as those challenged 2 months post-vaccination, with no vaccinated animals succumbing to WNV challenge. These results indicate that RepliVAX is capable of inducing durable protective immunity after a single dose.",

keywords = "Antibody, Hamster, Live-attenuated vaccine",

author = "Widman, {Douglas G.} and Tomohiro Ishikawa and Winkelmann, {Evandro R.} and Ernesto Infante and Nigel Bourne and Mason, {Peter W.}",

note = "Funding Information: This work was supported by a grant from NIAID to PWM through the Western Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research (NIH grant number U54 AI057156) and DGW was supported by a James W. McLaughlin fellowship. ",

year = "2009",

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doi = "10.1016/j.vaccine.2009.07.016",

language = "English (US)",

volume = "27",

pages = "5550--5553",

journal = "Vaccine",

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T1 - RepliVAX WN, a single-cycle flavivirus vaccine to prevent West Nile disease, elicits durable protective immunity in hamsters

AU - Widman, Douglas G.

AU - Ishikawa, Tomohiro

AU - Winkelmann, Evandro R.

AU - Infante, Ernesto

AU - Bourne, Nigel

AU - Mason, Peter W.

N1 - Funding Information: This work was supported by a grant from NIAID to PWM through the Western Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research (NIH grant number U54 AI057156) and DGW was supported by a James W. McLaughlin fellowship.

PY - 2009/9/18

Y1 - 2009/9/18

N2 - West Nile virus (WNV) causes significant disease, yet no vaccines exist to prevent WN disease in humans. We have previously reported that RepliVAX WN is a safe and efficacious vaccine in mouse and hamster models of WN disease. Here, we report that vaccination of hamsters with RepliVAX WN induces antibody responses that remain stable for at least 6 months. Furthermore, animals challenged with virulent WNV 6 months after vaccination were protected from disease as well as those challenged 2 months post-vaccination, with no vaccinated animals succumbing to WNV challenge. These results indicate that RepliVAX is capable of inducing durable protective immunity after a single dose.

AB - West Nile virus (WNV) causes significant disease, yet no vaccines exist to prevent WN disease in humans. We have previously reported that RepliVAX WN is a safe and efficacious vaccine in mouse and hamster models of WN disease. Here, we report that vaccination of hamsters with RepliVAX WN induces antibody responses that remain stable for at least 6 months. Furthermore, animals challenged with virulent WNV 6 months after vaccination were protected from disease as well as those challenged 2 months post-vaccination, with no vaccinated animals succumbing to WNV challenge. These results indicate that RepliVAX is capable of inducing durable protective immunity after a single dose.

KW - Antibody

KW - Hamster

KW - Live-attenuated vaccine

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JF - Vaccine

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ER -

RepliVAX WN, a single-cycle flavivirus vaccine to prevent West Nile disease, elicits durable protective immunity in hamsters

Abstract

Keywords

ASJC Scopus subject areas

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