TY - JOUR
T1 - Repair of pyrimidine dimers in radiation-sensitive mutants rad3, rad4, rad6 and rad9 of Saccharomyces cerevisiae
AU - Prakash, Louise
N1 - Funding Information:
This investigation was supported in part by the U.S. Public Health Service (research grant GM19261 from the National Institutes of Health) and in part by the U.S. Energy Research and Development Administration at the University of Rochester Biomedical and Environmental Research Project. This paper has been designated report No. UR-3490-965.
Funding Information:
The author was supported in part by a U.S. Public Health Service career development award (GM-00004) from the National Institutes of Health.
PY - 1977/10
Y1 - 1977/10
N2 - The ability to remove ultraviolet (UV)-induced pyrimidine dimers was examined in four radiation-sensitive mutants of Saccharomyces cerevisiae. The susceptibility of DNA from irradiated cells to nicking by either the T4 UV-endonuclease or an endonuclease activity found in crude extracts of Micrococcus luteus was used to measure the presence of dimers in DNA. The rad3 and rad4 mutants are shown to be defective in dimer excision whereas the rad6 and rad9 mutants are proficient in dimer excision.
AB - The ability to remove ultraviolet (UV)-induced pyrimidine dimers was examined in four radiation-sensitive mutants of Saccharomyces cerevisiae. The susceptibility of DNA from irradiated cells to nicking by either the T4 UV-endonuclease or an endonuclease activity found in crude extracts of Micrococcus luteus was used to measure the presence of dimers in DNA. The rad3 and rad4 mutants are shown to be defective in dimer excision whereas the rad6 and rad9 mutants are proficient in dimer excision.
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U2 - 10.1016/0027-5107(77)90038-0
DO - 10.1016/0027-5107(77)90038-0
M3 - Article
C2 - 335235
AN - SCOPUS:0017672042
SN - 0027-5107
VL - 45
SP - 13
EP - 20
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1
ER -