Regulation of the nitric oxide synthase-nitric oxide-cGMP pathway in rat mesenteric endothelial cells

Andreas Papapetropoulos, Stavroula Andreopoulos, Carolyn Y. Go, Azizul Hoque, Leslie C. Fuchs, John D. Catravas

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Most of the available data on the nitric oxide (NO) pathway in the vasculature is derived from studies performed with cells isolated from conduit arteries. We investigated the expression and regulation of components of the NO synthase (NOS)-NO-cGMP pathway in endothelial cells from the mesenteric vascular bed. Basally, or in response to bradykinin, cultured mesenteric endothelial cells (MEC) do not release NO and do not express endothelial NOS protein. MEC treated with cytokines, but not untreated cells, express inducible NOS (iNOS) mRNA and protein, increase nitrite release, and stimulate cGMP accumulation in reporter smooth muscle cells. Pretreatment of MEC with genistein abolished the cytokine-induced iNOS expression. On the other hand, exposure of MEC to the microtubule depolymerizing agent colchicine did not affect the cytokine-induced increase in nitrite formation and iNOS protein expression, whereas it inhibited the induction of iNOS in smooth muscle cells. Collectively, our findings demonstrate that MEC do not express endothelial NOS but respond to inflammatory stimuli by expressing iNOS, a process that is blocked by tyrosine kinase inhibition but not by microtubule depolymerization.

Original languageEnglish (US)
Pages (from-to)2553-2560
Number of pages8
JournalJournal of Applied Physiology
Issue number6
StatePublished - 2001
Externally publishedYes


  • Endothelium
  • Guanosine 3′,5′-cyclic monophosphate
  • Guanylyl cyclase
  • Microtubules

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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