Regulation of dynamin oligomerization in cells: The role of dynamin-actin interactions and its GTPase activity

Changkyu Gu, Joann Chang, Valentina A. Shchedrina, Vincent A. Pham, John H. Hartwig, Worawit Suphamungmee, William Lehman, Bradley T. Hyman, Brian J. Bacskai, Sanja Sever

Research output: Contribution to journalArticlepeer-review

Abstract

Dynamin is a 96-kDa protein that has multiple oligomerization states that influence its GTPase activity. A number of different dynamin effectors, including lipids, actin filaments, and SH3-domain-containing proteins, have been implicated in the regulation of dynamin oligomerization, though their roles in influencing dynamin oligomerization have been studied predominantly in vitro using recombinant proteins. Here, we identify higher order dynamin oligomers such as rings and helices in vitro and in live cells using fluorescence lifetime imaging microscopy (FLIM). FLIM detected GTP- and actin-dependent dynamin oligomerization at distinct cellular sites, including the cell membrane and transition zones where cortical actin transitions into stress fibers. Our study identifies a major role for direct dynamin-actin interactions and dynamin's GTPase activity in the regulation of dynamin oligomerization in cells.

Original languageEnglish (US)
Pages (from-to)819-838
Number of pages20
JournalTraffic
Volume15
Issue number8
DOIs
StatePublished - Aug 2014
Externally publishedYes

Keywords

  • Actin
  • Dynamin
  • Dynamin oligomerization
  • Fluorescence lifetime imaging microscopy

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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