Reduced mobilization of CD34+ stem cells in advanced human immunodeficiency virus type 1 disease

Robert T. Schooley; Jeannette Mladenovic; Anne Sevin; Simon Chiu; Steven A. Miles; Roger J. Pomerantz; Thomas B. Campbell; Dawn Bell; Daniel Ambruso; Robbie Wong; Alan Landay; Robert W. Coombs; Fox Lawrence; Malek Kamoun; Janice Jacovini

doi:10.1086/315168

Reduced mobilization of CD34⁺ stem cells in advanced human immunodeficiency virus type 1 disease

Robert T. Schooley, Jeannette Mladenovic, Anne Sevin, Simon Chiu, Steven A. Miles, Roger J. Pomerantz, Thomas B. Campbell, Dawn Bell, Daniel Ambruso, Robbie Wong, Alan Landay, Robert W. Coombs, Fox Lawrence, Malek Kamoun, Janice Jacovini

Research output: Contribution to journal › Article › peer-review

Abstract

Granulocyte colony-stimulating factor (r-met Hu G-CSF; filgrastim; 10 μg/kg/day for 7 days) was used to mobilize CD34⁺stem cells into the peripheral blood of human immunodeficiency virus type 1 (HIV-1)-infected individuals and a group of HIV-1-uninfected donors as a measure of immunologic reserve in HIV-1-infected people. G-CSF mobilized CD34⁺ cells of HIV-1-infected individuals with cell counts >500 CD4⁺ cells/mm³, as well as in HIV-1-uninfected donors. In contrast, CD34⁺ cell mobilization was significantly blunted in HIV-1-infected individuals with cell counts <500 CD4⁺ cells/mm³ (<200 cell days vs. >650 cell days, P < .0005, compared with the >500 CD4⁺ cell cohort). At least 1.75 x 10⁷ CD34⁺ cells were harvested by leukapheresis from patients in each study cohort. CD34⁺ cell viability and the ability to differentiate precursor cells into myeloid and erythroid progenitor cells were not affected by HIV-1 infection.

Original language	English (US)
Pages (from-to)	148-157
Number of pages	10
Journal	Journal of Infectious Diseases
Volume	181
Issue number	1
DOIs	https://doi.org/10.1086/315168
State	Published - 2000
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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10.1086/315168

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Schooley, R. T., Mladenovic, J., Sevin, A., Chiu, S., Miles, S. A., Pomerantz, R. J., Campbell, T. B., Bell, D., Ambruso, D., Wong, R., Landay, A., Coombs, R. W., Lawrence, F., Kamoun, M., & Jacovini, J. (2000). Reduced mobilization of CD34⁺ stem cells in advanced human immunodeficiency virus type 1 disease. Journal of Infectious Diseases, 181(1), 148-157. https://doi.org/10.1086/315168

Schooley, RT, Mladenovic, J, Sevin, A, Chiu, S, Miles, SA, Pomerantz, RJ, Campbell, TB, Bell, D, Ambruso, D, Wong, R, Landay, A, Coombs, RW, Lawrence, F, Kamoun, M & Jacovini, J 2000, 'Reduced mobilization of CD34⁺ stem cells in advanced human immunodeficiency virus type 1 disease', Journal of Infectious Diseases, vol. 181, no. 1, pp. 148-157. https://doi.org/10.1086/315168

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title = "Reduced mobilization of CD34+ stem cells in advanced human immunodeficiency virus type 1 disease",

abstract = "Granulocyte colony-stimulating factor (r-met Hu G-CSF; filgrastim; 10 μg/kg/day for 7 days) was used to mobilize CD34+stem cells into the peripheral blood of human immunodeficiency virus type 1 (HIV-1)-infected individuals and a group of HIV-1-uninfected donors as a measure of immunologic reserve in HIV-1-infected people. G-CSF mobilized CD34+ cells of HIV-1-infected individuals with cell counts >500 CD4+ cells/mm3, as well as in HIV-1-uninfected donors. In contrast, CD34+ cell mobilization was significantly blunted in HIV-1-infected individuals with cell counts <500 CD4+ cells/mm3 (<200 cell days vs. >650 cell days, P < .0005, compared with the >500 CD4+ cell cohort). At least 1.75 x 107 CD34+ cells were harvested by leukapheresis from patients in each study cohort. CD34+ cell viability and the ability to differentiate precursor cells into myeloid and erythroid progenitor cells were not affected by HIV-1 infection.",

author = "Schooley, {Robert T.} and Jeannette Mladenovic and Anne Sevin and Simon Chiu and Miles, {Steven A.} and Pomerantz, {Roger J.} and Campbell, {Thomas B.} and Dawn Bell and Daniel Ambruso and Robbie Wong and Alan Landay and Coombs, {Robert W.} and Fox Lawrence and Malek Kamoun and Janice Jacovini",

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doi = "10.1086/315168",

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AU - Pomerantz, Roger J.

AU - Campbell, Thomas B.

AU - Bell, Dawn

AU - Ambruso, Daniel

AU - Wong, Robbie

AU - Landay, Alan

AU - Coombs, Robert W.

AU - Lawrence, Fox

AU - Kamoun, Malek

AU - Jacovini, Janice

PY - 2000

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AB - Granulocyte colony-stimulating factor (r-met Hu G-CSF; filgrastim; 10 μg/kg/day for 7 days) was used to mobilize CD34+stem cells into the peripheral blood of human immunodeficiency virus type 1 (HIV-1)-infected individuals and a group of HIV-1-uninfected donors as a measure of immunologic reserve in HIV-1-infected people. G-CSF mobilized CD34+ cells of HIV-1-infected individuals with cell counts >500 CD4+ cells/mm3, as well as in HIV-1-uninfected donors. In contrast, CD34+ cell mobilization was significantly blunted in HIV-1-infected individuals with cell counts <500 CD4+ cells/mm3 (<200 cell days vs. >650 cell days, P < .0005, compared with the >500 CD4+ cell cohort). At least 1.75 x 107 CD34+ cells were harvested by leukapheresis from patients in each study cohort. CD34+ cell viability and the ability to differentiate precursor cells into myeloid and erythroid progenitor cells were not affected by HIV-1 infection.

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Reduced mobilization of CD34⁺ stem cells in advanced human immunodeficiency virus type 1 disease

Abstract

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