Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas

Aaron C. Brault; Stanley A. Langevin; Wanichaya N. Ramey; Ying Fang; David W.C. Beasley; Christopher M. Barker; Todd A. Sanders; William K. Reisen; Alan D.T. Barrett; Richard A. Bowen

doi:10.4269/ajtmh.2011.10-0439

Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas

Aaron C. Brault, Stanley A. Langevin, Wanichaya N. Ramey, Ying Fang, David W.C. Beasley, Christopher M. Barker, Todd A. Sanders, William K. Reisen, Alan D.T. Barrett, Richard A. Bowen

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.

Original language	English (US)
Pages (from-to)	758-767
Number of pages	10
Journal	American Journal of Tropical Medicine and Hygiene
Volume	85
Issue number	4
DOIs	https://doi.org/10.4269/ajtmh.2011.10-0439
State	Published - Oct 2011

ASJC Scopus subject areas

Parasitology
Infectious Diseases
Virology

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title = "Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas",

abstract = "A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.",

author = "Brault, {Aaron C.} and Langevin, {Stanley A.} and Ramey, {Wanichaya N.} and Ying Fang and Beasley, {David W.C.} and Barker, {Christopher M.} and Sanders, {Todd A.} and Reisen, {William K.} and Barrett, {Alan D.T.} and Bowen, {Richard A.}",

year = "2011",

month = oct,

doi = "10.4269/ajtmh.2011.10-0439",

language = "English (US)",

volume = "85",

pages = "758--767",

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AU - Brault, Aaron C.

AU - Langevin, Stanley A.

AU - Ramey, Wanichaya N.

AU - Fang, Ying

AU - Beasley, David W.C.

AU - Barker, Christopher M.

AU - Sanders, Todd A.

AU - Reisen, William K.

AU - Barrett, Alan D.T.

AU - Bowen, Richard A.

PY - 2011/10

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N2 - A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.

AB - A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.

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Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas

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