TY - JOUR
T1 - Rapid neurite formation in a human cortical neuronal cell line
AU - Dunn, K. J.
AU - Perez-Polo, J. R.
AU - Wood, T. G.
N1 - Funding Information:
Acknowledgements-Supported in part by a grant from the Moody Foundation. Thanks to Donna Masters for manuscript preparation.
PY - 1996/2
Y1 - 1996/2
N2 - The subclone HCN-1 was derived from parental cell lines from cortical tissue of a patient with unilateral megalencephaly growth and immunochemistry staining characteristics. As we and others have shown, HCN-1A cells can be induced to differentiate to a neuronal-like morphology. HCN-1A cells stain positively for neurofilament, neuron-specific enolase and the low-affinity neurotrophin receptors, p75(NGFR), but not for myelin basic protein, S-100, or glial fibrillary acidic protein (GFAP). HCN-1A cells also stain positively for gamma-aminobutyric acid and glutamate. In the present study, we examine the effects of cell density on the requirements for efficient induction of differentiation of HCN-1A cells and analyze the time course of this induction and its reversion. We also characterize the changes in cytoskeletal proteins of HCN-1A cells in response to their differentiation neuronal phenotype.
AB - The subclone HCN-1 was derived from parental cell lines from cortical tissue of a patient with unilateral megalencephaly growth and immunochemistry staining characteristics. As we and others have shown, HCN-1A cells can be induced to differentiate to a neuronal-like morphology. HCN-1A cells stain positively for neurofilament, neuron-specific enolase and the low-affinity neurotrophin receptors, p75(NGFR), but not for myelin basic protein, S-100, or glial fibrillary acidic protein (GFAP). HCN-1A cells also stain positively for gamma-aminobutyric acid and glutamate. In the present study, we examine the effects of cell density on the requirements for efficient induction of differentiation of HCN-1A cells and analyze the time course of this induction and its reversion. We also characterize the changes in cytoskeletal proteins of HCN-1A cells in response to their differentiation neuronal phenotype.
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U2 - 10.1016/0736-5748(95)00073-9
DO - 10.1016/0736-5748(95)00073-9
M3 - Article
C2 - 8779309
AN - SCOPUS:0029877321
SN - 0736-5748
VL - 14
SP - 61
EP - 68
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
IS - 1
ER -