Rapid molecular strategy for orbivirus detection and characterization

Gustavo Palacios; Chris Cowled; Ana V. Bussetti; Nazir Savji; Richard Weir; Ivan Wick; Amelia Travassos Da Rosa; Charles H. Calisher; Robert B. Tesh; David Boyle; W. Ian Lipkin

doi:10.1128/JCM.00337-11

Rapid molecular strategy for orbivirus detection and characterization

Gustavo Palacios, Chris Cowled, Ana V. Bussetti, Nazir Savji, Richard Weir, Ivan Wick, Amelia Travassos Da Rosa, Charles H. Calisher, Robert B. Tesh, David Boyle, W. Ian Lipkin

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

Original language	English (US)
Pages (from-to)	2314-2317
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00337-11
State	Published - Jun 2011
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.00337-11

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abstract = "Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.",

author = "Gustavo Palacios and Chris Cowled and Bussetti, {Ana V.} and Nazir Savji and Richard Weir and Ivan Wick and {Da Rosa}, {Amelia Travassos} and Calisher, {Charles H.} and Tesh, {Robert B.} and David Boyle and Lipkin, {W. Ian}",

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AU - Palacios, Gustavo

AU - Cowled, Chris

AU - Bussetti, Ana V.

AU - Savji, Nazir

AU - Weir, Richard

AU - Wick, Ivan

AU - Da Rosa, Amelia Travassos

AU - Calisher, Charles H.

AU - Tesh, Robert B.

AU - Boyle, David

AU - Lipkin, W. Ian

PY - 2011/6

Y1 - 2011/6

N2 - Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

AB - Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

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U2 - 10.1128/JCM.00337-11

DO - 10.1128/JCM.00337-11

M3 - Article

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JO - Journal of Clinical Microbiology

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ER -

Rapid molecular strategy for orbivirus detection and characterization

Abstract

ASJC Scopus subject areas

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