Rapid isolation of total RNA from mammalian tissues

Mark Emmett; Barbara Petrack

doi:10.1016/0003-2697(88)90069-3

Rapid isolation of total RNA from mammalian tissues

Mark Emmett, Barbara Petrack

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chromatography. Total RNAs were precipitated with ethanol and sodium acetate. The RNAs isolated were intact and suitable for mRNA quantitation via Northern blot or slot-blot analyses. This procedure isolates total RNAs in high yield and purity, without CsCl ultracentrifugation, and is especially useful when mRNAs must be quantitated from many samples.

Original language	English (US)
Pages (from-to)	658-661
Number of pages	4
Journal	Analytical Biochemistry
Volume	174
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(88)90069-3
State	Published - Nov 1 1988
Externally published	Yes

Keywords

RNA isolation
RNA purification
Sephadex columns
total RNA

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(88)90069-3

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N2 - A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chromatography. Total RNAs were precipitated with ethanol and sodium acetate. The RNAs isolated were intact and suitable for mRNA quantitation via Northern blot or slot-blot analyses. This procedure isolates total RNAs in high yield and purity, without CsCl ultracentrifugation, and is especially useful when mRNAs must be quantitated from many samples.

AB - A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chromatography. Total RNAs were precipitated with ethanol and sodium acetate. The RNAs isolated were intact and suitable for mRNA quantitation via Northern blot or slot-blot analyses. This procedure isolates total RNAs in high yield and purity, without CsCl ultracentrifugation, and is especially useful when mRNAs must be quantitated from many samples.

KW - RNA isolation

KW - RNA purification

KW - Sephadex columns

KW - total RNA

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Rapid isolation of total RNA from mammalian tissues

Abstract

Keywords

ASJC Scopus subject areas

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