Rapid diagnosis of rift valley fever: A comparison of methods for the direct detection of viral antigen in human sera

J. Meegan; B. Le Guenno; T. Ksiazek; A. Jouan; F. Knauert; J. P. Digoutte; C. J. Peters

doi:10.1016/S0923-2516(89)80085-8

Rapid diagnosis of rift valley fever: A comparison of methods for the direct detection of viral antigen in human sera

J. Meegan, B. Le Guenno, T. Ksiazek, A. Jouan, F. Knauert, J. P. Digoutte, C. J. Peters

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Human sera collected during the 1987 Rift Valley fever (RVF) epidemic in the Senegal River basin were analysed using three enzyme immunoassays to establish the best method for rapid diagnosis of RVF. A biotin-avidinenhanced antigen detection method utilizing monoclonal antibodies proved most sensitive. Eighty-two viremic human sera were tested, and this assay detected antigen in 29.3 % of the samples.

Original language	English (US)
Pages (from-to)	59-65
Number of pages	7
Journal	Research in Virology
Volume	140
Issue number	C
DOIs	https://doi.org/10.1016/S0923-2516(89)80085-8
State	Published - 1989
Externally published	Yes

Keywords

EIA, Diagnosis, Senegal
RVF virus, mAb

ASJC Scopus subject areas

Immunology
Virology

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10.1016/S0923-2516(89)80085-8

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title = "Rapid diagnosis of rift valley fever: A comparison of methods for the direct detection of viral antigen in human sera",

abstract = "Human sera collected during the 1987 Rift Valley fever (RVF) epidemic in the Senegal River basin were analysed using three enzyme immunoassays to establish the best method for rapid diagnosis of RVF. A biotin-avidinenhanced antigen detection method utilizing monoclonal antibodies proved most sensitive. Eighty-two viremic human sera were tested, and this assay detected antigen in 29.3 % of the samples.",

keywords = "EIA, Diagnosis, Senegal, RVF virus, mAb",

author = "J. Meegan and Guenno, {B. Le} and T. Ksiazek and A. Jouan and F. Knauert and Digoutte, {J. P.} and Peters, {C. J.}",

year = "1989",

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language = "English (US)",

volume = "140",

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T1 - Rapid diagnosis of rift valley fever

T2 - A comparison of methods for the direct detection of viral antigen in human sera

AU - Meegan, J.

AU - Guenno, B. Le

AU - Ksiazek, T.

AU - Jouan, A.

AU - Knauert, F.

AU - Digoutte, J. P.

AU - Peters, C. J.

PY - 1989

Y1 - 1989

N2 - Human sera collected during the 1987 Rift Valley fever (RVF) epidemic in the Senegal River basin were analysed using three enzyme immunoassays to establish the best method for rapid diagnosis of RVF. A biotin-avidinenhanced antigen detection method utilizing monoclonal antibodies proved most sensitive. Eighty-two viremic human sera were tested, and this assay detected antigen in 29.3 % of the samples.

AB - Human sera collected during the 1987 Rift Valley fever (RVF) epidemic in the Senegal River basin were analysed using three enzyme immunoassays to establish the best method for rapid diagnosis of RVF. A biotin-avidinenhanced antigen detection method utilizing monoclonal antibodies proved most sensitive. Eighty-two viremic human sera were tested, and this assay detected antigen in 29.3 % of the samples.

KW - EIA, Diagnosis, Senegal

KW - RVF virus, mAb

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SN - 0923-2516

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SP - 59

EP - 65

JO - Research in Virology

JF - Research in Virology

IS - C

ER -

Rapid diagnosis of rift valley fever: A comparison of methods for the direct detection of viral antigen in human sera

Abstract

Keywords

ASJC Scopus subject areas

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