TY - JOUR
T1 - Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography
AU - Wang, Xiao Ming
AU - Yang, Min
AU - Guan, Shu Hong
AU - Liu, Rong Xia
AU - Xia, Jia Meng
AU - Bi, Kai Shun
AU - Guo, De An
N1 - Funding Information:
We thank the Shanghai Commission of Science and Technology (04DZ19848), National Administration of Traditional Chinese Medicine of China (2004ZX01) and Green Valley Pharmaceutical Group for financial support of this work.
PY - 2006/6/7
Y1 - 2006/6/7
N2 - A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.
AB - A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.
KW - Ganoderma lucidum
KW - Reversed-phase high performance liquid chromatography
KW - Triterpenoids
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U2 - 10.1016/j.jpba.2006.01.053
DO - 10.1016/j.jpba.2006.01.053
M3 - Article
C2 - 16530372
AN - SCOPUS:33646415921
SN - 0731-7085
VL - 41
SP - 838
EP - 844
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 3
ER -