Quantitation of cytoplasmic fibrinogen in rat liver cells

Sau Wah Kwan; Gerald M. Fuller; Martha A. Krautter; Julius H. van Bavel; Randall M. Goldblum

doi:10.1016/0003-2697(77)90062-8

Quantitation of cytoplasmic fibrinogen in rat liver cells

Sau Wah Kwan, Gerald M. Fuller, Martha A. Krautter, Julius H. van Bavel, Randall M. Goldblum

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Following an inflammatory response, the fibrinogen content of postmicrosomal supernatants from rat liver was quantitated using an enzyme-linked immunosorbent assay. A fivefold increase in intracellular fibrinogen was observed in the liver cells of turpentine-stimulated animals. The utility of the enzyme-linked immunosorbent assay for investigating the induction of fibrinogen synthesis was demonstrated.

Original language	English (US)
Pages (from-to)	589-596
Number of pages	8
Journal	Analytical Biochemistry
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(77)90062-8
State	Published - Dec 1977
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{018d04f77b1a410191449b9e0a81f9bf,

title = "Quantitation of cytoplasmic fibrinogen in rat liver cells",

abstract = "Following an inflammatory response, the fibrinogen content of postmicrosomal supernatants from rat liver was quantitated using an enzyme-linked immunosorbent assay. A fivefold increase in intracellular fibrinogen was observed in the liver cells of turpentine-stimulated animals. The utility of the enzyme-linked immunosorbent assay for investigating the induction of fibrinogen synthesis was demonstrated.",

author = "Kwan, {Sau Wah} and Fuller, {Gerald M.} and Krautter, {Martha A.} and {van Bavel}, {Julius H.} and Goldblum, {Randall M.}",

note = "Funding Information: The authors wish to thank Ms. Shirley Sidoti for her excellent secretarial assistance. This study was supported by Grant HL 16445 from the Heart, Lung, and Blood Institute of the National Institutes of Health and by a Research Career Development Award, HL 00162, to GMF. JvB is the recipient of a McLaughlin Predoctoral Fellowship. We also wish to acknowledge Grant DHEW 5SOl RR(606)05427, to RMG.",

year = "1977",

month = dec,

doi = "10.1016/0003-2697(77)90062-8",

language = "English (US)",

volume = "83",

pages = "589--596",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Quantitation of cytoplasmic fibrinogen in rat liver cells

AU - Kwan, Sau Wah

AU - Fuller, Gerald M.

AU - Krautter, Martha A.

AU - van Bavel, Julius H.

AU - Goldblum, Randall M.

N1 - Funding Information: The authors wish to thank Ms. Shirley Sidoti for her excellent secretarial assistance. This study was supported by Grant HL 16445 from the Heart, Lung, and Blood Institute of the National Institutes of Health and by a Research Career Development Award, HL 00162, to GMF. JvB is the recipient of a McLaughlin Predoctoral Fellowship. We also wish to acknowledge Grant DHEW 5SOl RR(606)05427, to RMG.

PY - 1977/12

Y1 - 1977/12

N2 - Following an inflammatory response, the fibrinogen content of postmicrosomal supernatants from rat liver was quantitated using an enzyme-linked immunosorbent assay. A fivefold increase in intracellular fibrinogen was observed in the liver cells of turpentine-stimulated animals. The utility of the enzyme-linked immunosorbent assay for investigating the induction of fibrinogen synthesis was demonstrated.

AB - Following an inflammatory response, the fibrinogen content of postmicrosomal supernatants from rat liver was quantitated using an enzyme-linked immunosorbent assay. A fivefold increase in intracellular fibrinogen was observed in the liver cells of turpentine-stimulated animals. The utility of the enzyme-linked immunosorbent assay for investigating the induction of fibrinogen synthesis was demonstrated.

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DO - 10.1016/0003-2697(77)90062-8

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SP - 589

EP - 596

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Quantitation of cytoplasmic fibrinogen in rat liver cells

Abstract

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