TY - JOUR
T1 - Purification and X-ray crystallographic analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from Mycobacterium smegmatis
AU - Fan, Shanghua
AU - Li, Defeng
AU - Fleming, Joy
AU - Hong, Yuan
AU - Chen, Tao
AU - Zhou, Lin
AU - Bi, Lijun
AU - Wang, Dacheng
AU - Zhang, Xianen
AU - Chen, Guanjun
N1 - Publisher Copyright:
© 2014 International Union of Crystallography. All rights reserved.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - 7-Keto-8-aminopelargonic acid synthase (KAPA synthase; BioF) is an essential enzyme for mycobacterial growth that catalyses the first committed step in the biotin-synthesis pathway. It is a pyridoxal 5′-phosphate (PLP)-dependent enzyme and is a potential drug target. Here, the cloning, expression, purification and crystallization of KAPA synthase from Mycobacterium smegmatis (MsBioF) and the characterization of MsBioF crystals using X-ray diffraction are described. The crystals diffracted to 2.3 Å resolution and belonged to the monoclinic space group P21, with unit-cell parameters a = 70.88, b = 91.68, c = 109.84 Å, β = 97.8°. According to the molecular weight of MsBioF, the unit-cell parameters and the self-rotation function map, four molecules are present in each asymmetric unit with a V M value of 2.06 Å3 Da-1 and a solvent content of 40.20%.
AB - 7-Keto-8-aminopelargonic acid synthase (KAPA synthase; BioF) is an essential enzyme for mycobacterial growth that catalyses the first committed step in the biotin-synthesis pathway. It is a pyridoxal 5′-phosphate (PLP)-dependent enzyme and is a potential drug target. Here, the cloning, expression, purification and crystallization of KAPA synthase from Mycobacterium smegmatis (MsBioF) and the characterization of MsBioF crystals using X-ray diffraction are described. The crystals diffracted to 2.3 Å resolution and belonged to the monoclinic space group P21, with unit-cell parameters a = 70.88, b = 91.68, c = 109.84 Å, β = 97.8°. According to the molecular weight of MsBioF, the unit-cell parameters and the self-rotation function map, four molecules are present in each asymmetric unit with a V M value of 2.06 Å3 Da-1 and a solvent content of 40.20%.
KW - 7-keto-8-aminopelargonic acid (KAPA) synthase
KW - Mycobacterium smegmatis
KW - biotin-synthesis pathway
UR - http://www.scopus.com/inward/record.url?scp=84912521122&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84912521122&partnerID=8YFLogxK
U2 - 10.1107/S2053230X14018317
DO - 10.1107/S2053230X14018317
M3 - Article
C2 - 25286942
AN - SCOPUS:84912521122
SN - 1744-3091
VL - 70
SP - 1372
EP - 1375
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
ER -