Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab

Thomas James Smith; Elaine S. Chase

doi:10.1016/0042-6822(92)90235-H

Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab

Thomas James Smith, Elaine S. Chase

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 Å resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.

Original language	English (US)
Pages (from-to)	600-606
Number of pages	7
Journal	Virology
Volume	191
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(92)90235-H
State	Published - Dec 1992
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(92)90235-H

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title = "Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab",

abstract = "We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 {\AA} resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.",

author = "Smith, {Thomas James} and Chase, {Elaine S.}",

note = "Funding Information: We thank N. H. Olson and W. Lee for taking the electron microscope Images of Fig. 3a and 3b, respectively. This work was supported by an NIH Grant (GM 10704-29) to T.J.S.",

year = "1992",

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doi = "10.1016/0042-6822(92)90235-H",

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T1 - Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab

AU - Smith, Thomas James

AU - Chase, Elaine S.

N1 - Funding Information: We thank N. H. Olson and W. Lee for taking the electron microscope Images of Fig. 3a and 3b, respectively. This work was supported by an NIH Grant (GM 10704-29) to T.J.S.

PY - 1992/12

Y1 - 1992/12

N2 - We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 Å resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.

AB - We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0 Å resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.

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JF - Virology

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ER -

Purification and crystallization of intact human rhinovirus complexed with a neutralizing fab

Abstract

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