TY - JOUR
T1 - PTH-related protein modulates PC-3 prostate cancer cell adhesion and integrin subunit profile
AU - Shen, Xiaoli
AU - Falzon, Miriam
N1 - Funding Information:
We wish to thank Dr Rolf Konig for advice with FACS analysis, and Drs D. Konkel, P.K. Seitz, and M.T. Thomas for critical reading of the manuscript. This work was supported by NIH grant CA83940.
PY - 2003/1/31
Y1 - 2003/1/31
N2 - Parathyroid hormone-related protein (PTHrP), which has been localized in prostate cancer tissue and cell lines, plays a role in the development of bone metastases, a frequent complication in prostate cancer patients. Tumor cell adhesion to extracellular matrix (ECM) components is mediated via integrin subunits, and plays a major role in the invasion and metastasis of tumor cells. The present experiments examined the ability of PTHrP to influence adhesion of the human prostate cancer cell line PC-3 to several ECM proteins found in normal tissues. Clonal PC-3 cells induced to overexpress PTHrP by stable transfection with PTHrP complementary DNA showed significantly higher adhesion to collagen type 1, fibronectin, and laminin than control (empty vector-transfected) cells. PTHrP-overexpressing cells also exhibited higher expression of the α1, α5, α6, and β4 integrin subunits. These results suggest that PTHrP may play a role in prostate tumor invasion and metastasis by influencing cell adhesion to the ECM via upregulation of specific integrin subunits.
AB - Parathyroid hormone-related protein (PTHrP), which has been localized in prostate cancer tissue and cell lines, plays a role in the development of bone metastases, a frequent complication in prostate cancer patients. Tumor cell adhesion to extracellular matrix (ECM) components is mediated via integrin subunits, and plays a major role in the invasion and metastasis of tumor cells. The present experiments examined the ability of PTHrP to influence adhesion of the human prostate cancer cell line PC-3 to several ECM proteins found in normal tissues. Clonal PC-3 cells induced to overexpress PTHrP by stable transfection with PTHrP complementary DNA showed significantly higher adhesion to collagen type 1, fibronectin, and laminin than control (empty vector-transfected) cells. PTHrP-overexpressing cells also exhibited higher expression of the α1, α5, α6, and β4 integrin subunits. These results suggest that PTHrP may play a role in prostate tumor invasion and metastasis by influencing cell adhesion to the ECM via upregulation of specific integrin subunits.
KW - Extracellular matrix
KW - Integrins
KW - PC-3 (prostate cancer cells)
KW - Parathyroid hormone-related protein
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U2 - 10.1016/S0303-7207(02)00287-3
DO - 10.1016/S0303-7207(02)00287-3
M3 - Article
C2 - 12581888
AN - SCOPUS:0037474110
SN - 0303-7207
VL - 199
SP - 165
EP - 177
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -