@inbook{296b14644e994d4a82cac309f7551a32,
title = "Propagation and titration of west Nile virus on Vero cells",
abstract = "The propagation and titration of viruses are key virological techniques. Unlike other flaviviruses, such as the dengue viruses, West Nile virus (WNV) grows and plaques very efficiently on Vero cells, usually inducing strong cytopathic effect (CPE) and forming clear plaques. Here, we outline the steps for propagating WNV from culture supernatant stocks and homogenized organ/mosquito samples, as well as for determining virus titers in samples by serial-dilution plaque assay using neutral red or crystal violet stains.",
keywords = "Plaque assay, Vero cells, Virus propagation, Virus titration, West Nile virus",
author = "Alexander McAuley and Beasley, {David W.C.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3670-0_3",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "19--27",
booktitle = "Methods in Molecular Biology",
}