Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin

Vidyavathi Udupi; Heung Man Lee; Alexander Kurosky; George H. Greeley

doi:10.1016/S0167-0115(99)00061-0

Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin

Vidyavathi Udupi, Heung Man Lee, Alexander Kurosky, George H. Greeley

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	123-127
Number of pages	5
Journal	Regulatory Peptides
Volume	83
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-0115(99)00061-0
State	Published - Sep 15 1999
Externally published	Yes

Keywords

Antisense
Endoprotease
Peptide
Processing

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/S0167-0115(99)00061-0

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title = "Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin",

abstract = "The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Antisense, Endoprotease, Peptide, Processing",

author = "Vidyavathi Udupi and Lee, {Heung Man} and Alexander Kurosky and Greeley, {George H.}",

note = "Funding Information: Supported by a grant from the National Institutes of Health (PO1-DK35608).",

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AU - Udupi, Vidyavathi

AU - Lee, Heung Man

AU - Kurosky, Alexander

AU - Greeley, George H.

N1 - Funding Information: Supported by a grant from the National Institutes of Health (PO1-DK35608).

PY - 1999/9/15

Y1 - 1999/9/15

N2 - The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.

AB - The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (~95% reduction) and an 80% reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST. Copyright (C) 1999 Elsevier Science B.V.

KW - Antisense

KW - Endoprotease

KW - Peptide

KW - Processing

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ER -

Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin

Abstract

Keywords

ASJC Scopus subject areas

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