Production of IL-8, IL-17, IFN-gamma and IP-10 in human astrocytes correlates with alphavirus attenuation

Bi Hung Peng; Viktoriya Borisevich; Vsevolod L. Popov; Michele A. Zacks; D. Mark Estes; Gerald A. Campbell; Slobodan Paessler

doi:10.1016/j.vetmic.2012.11.021

Production of IL-8, IL-17, IFN-gamma and IP-10 in human astrocytes correlates with alphavirus attenuation

Bi Hung Peng, Viktoriya Borisevich, Vsevolod L. Popov, Michele A. Zacks, D. Mark Estes, Gerald A. Campbell, Slobodan Paessler

Pathology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Venezuelan equine encephalitis virus (VEEV) is an important, naturally emerging zoonotic pathogen. Recent outbreaks in Venezuela and Colombia in 1995 indicate that VEEV still poses a serious public health threat. Astrocytes may be target cells in human and mouse infection and they play an important role in repair through gliosis. In this study, we report that virulent VEEV efficiently infects cultured normal human astrocytes, three different murine astrocyte cell lines and astrocytes in the mouse brain. The attenuation of virus replication positively correlates with the increased levels of production of IL-8, IL-17, IFN-gamma and IP-10. In addition, VEEV infection induces release of basic fibroblast growth factor and production of potent chemokines such as RANTES and MIP-1-beta from cultured human astrocytes. This growth factor and cytokine profile modeled by astrocytes in vitro may contribute to both neuroprotection and repair and may play a role in leukocyte recruitment in vivo.

Original language	English (US)
Pages (from-to)	223-234
Number of pages	12
Journal	Veterinary Microbiology
Volume	163
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2012.11.021
State	Published - May 3 2013

Keywords

Alphavirus pathogenesis
Equine encephalitis
Viral encephalitis

ASJC Scopus subject areas

Microbiology
General Veterinary

Access to Document

10.1016/j.vetmic.2012.11.021

Cite this

@article{ac8b4019a1a744f19ac48e02b832d74d,

title = "Production of IL-8, IL-17, IFN-gamma and IP-10 in human astrocytes correlates with alphavirus attenuation",

abstract = "Venezuelan equine encephalitis virus (VEEV) is an important, naturally emerging zoonotic pathogen. Recent outbreaks in Venezuela and Colombia in 1995 indicate that VEEV still poses a serious public health threat. Astrocytes may be target cells in human and mouse infection and they play an important role in repair through gliosis. In this study, we report that virulent VEEV efficiently infects cultured normal human astrocytes, three different murine astrocyte cell lines and astrocytes in the mouse brain. The attenuation of virus replication positively correlates with the increased levels of production of IL-8, IL-17, IFN-gamma and IP-10. In addition, VEEV infection induces release of basic fibroblast growth factor and production of potent chemokines such as RANTES and MIP-1-beta from cultured human astrocytes. This growth factor and cytokine profile modeled by astrocytes in vitro may contribute to both neuroprotection and repair and may play a role in leukocyte recruitment in vivo.",

keywords = "Alphavirus pathogenesis, Equine encephalitis, Viral encephalitis",

author = "Peng, {Bi Hung} and Viktoriya Borisevich and Popov, {Vsevolod L.} and Zacks, {Michele A.} and Estes, {D. Mark} and Campbell, {Gerald A.} and Slobodan Paessler",

note = "Funding Information: This work was supported by a grants from the National Institute of Allergy and Infectious Diseases through the Galveston National Laboratory Operations, Advanced Veterinary Services Core ( UC7 AI070083 ). S. Paessler was supported by National Institutes of Health K08 Award ( A1059491 ) and faculty support provided by the Institute for Human Infections and Immunity at UTMB . We thank Jenna Linde for outstanding assistance with data entry and preparation of figures/manuscript.",

year = "2013",

month = may,

day = "3",

doi = "10.1016/j.vetmic.2012.11.021",

language = "English (US)",

volume = "163",

pages = "223--234",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier B.V.",

number = "3-4",

}

TY - JOUR

T1 - Production of IL-8, IL-17, IFN-gamma and IP-10 in human astrocytes correlates with alphavirus attenuation

AU - Peng, Bi Hung

AU - Borisevich, Viktoriya

AU - Popov, Vsevolod L.

AU - Zacks, Michele A.

AU - Estes, D. Mark

AU - Campbell, Gerald A.

AU - Paessler, Slobodan

N1 - Funding Information: This work was supported by a grants from the National Institute of Allergy and Infectious Diseases through the Galveston National Laboratory Operations, Advanced Veterinary Services Core ( UC7 AI070083 ). S. Paessler was supported by National Institutes of Health K08 Award ( A1059491 ) and faculty support provided by the Institute for Human Infections and Immunity at UTMB . We thank Jenna Linde for outstanding assistance with data entry and preparation of figures/manuscript.

PY - 2013/5/3

Y1 - 2013/5/3

N2 - Venezuelan equine encephalitis virus (VEEV) is an important, naturally emerging zoonotic pathogen. Recent outbreaks in Venezuela and Colombia in 1995 indicate that VEEV still poses a serious public health threat. Astrocytes may be target cells in human and mouse infection and they play an important role in repair through gliosis. In this study, we report that virulent VEEV efficiently infects cultured normal human astrocytes, three different murine astrocyte cell lines and astrocytes in the mouse brain. The attenuation of virus replication positively correlates with the increased levels of production of IL-8, IL-17, IFN-gamma and IP-10. In addition, VEEV infection induces release of basic fibroblast growth factor and production of potent chemokines such as RANTES and MIP-1-beta from cultured human astrocytes. This growth factor and cytokine profile modeled by astrocytes in vitro may contribute to both neuroprotection and repair and may play a role in leukocyte recruitment in vivo.

AB - Venezuelan equine encephalitis virus (VEEV) is an important, naturally emerging zoonotic pathogen. Recent outbreaks in Venezuela and Colombia in 1995 indicate that VEEV still poses a serious public health threat. Astrocytes may be target cells in human and mouse infection and they play an important role in repair through gliosis. In this study, we report that virulent VEEV efficiently infects cultured normal human astrocytes, three different murine astrocyte cell lines and astrocytes in the mouse brain. The attenuation of virus replication positively correlates with the increased levels of production of IL-8, IL-17, IFN-gamma and IP-10. In addition, VEEV infection induces release of basic fibroblast growth factor and production of potent chemokines such as RANTES and MIP-1-beta from cultured human astrocytes. This growth factor and cytokine profile modeled by astrocytes in vitro may contribute to both neuroprotection and repair and may play a role in leukocyte recruitment in vivo.

KW - Alphavirus pathogenesis

KW - Equine encephalitis

KW - Viral encephalitis

UR - http://www.scopus.com/inward/record.url?scp=84875397849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875397849&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2012.11.021

DO - 10.1016/j.vetmic.2012.11.021

M3 - Article

C2 - 23428380

AN - SCOPUS:84875397849

SN - 0378-1135

VL - 163

SP - 223

EP - 234

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 3-4

ER -

Production of IL-8, IL-17, IFN-gamma and IP-10 in human astrocytes correlates with alphavirus attenuation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this