Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked

Jie Yang; Xuesong Liu; Kapil Bhalla; Caryn Naekyung Kim; Ana Maria Ibrado; Jiyang Cai; Tsung I. Peng; Dean P. Jones; Xiaodong Wang

doi:10.1126/science.275.5303.1129

Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked

Jie Yang, Xuesong Liu, Kapil Bhalla, Caryn Naekyung Kim, Ana Maria Ibrado, Jiyang Cai, Tsung I. Peng, Dean P. Jones, Xiaodong Wang

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Abstract

Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.

Original language	English (US)
Pages (from-to)	1129-1132
Number of pages	4
Journal	Science
Volume	275
Issue number	5303
DOIs	https://doi.org/10.1126/science.275.5303.1129
State	Published - Feb 21 1997
Externally published	Yes

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title = "Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked",

abstract = "Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.",

author = "Jie Yang and Xuesong Liu and Kapil Bhalla and Kim, {Caryn Naekyung} and Ibrado, {Ana Maria} and Jiyang Cai and Peng, {Tsung I.} and Jones, {Dean P.} and Xiaodong Wang",

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T1 - Prevention of apoptosis by Bcl-2

T2 - Release of cytochrome c from mitochondria blocked

AU - Yang, Jie

AU - Liu, Xuesong

AU - Bhalla, Kapil

AU - Kim, Caryn Naekyung

AU - Ibrado, Ana Maria

AU - Cai, Jiyang

AU - Peng, Tsung I.

AU - Jones, Dean P.

AU - Wang, Xiaodong

PY - 1997/2/21

Y1 - 1997/2/21

N2 - Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.

AB - Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.

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Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked

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