TY - CHAP
T1 - Preparation and characterization of tau oligomer strains
AU - Sengupta, Urmi
AU - Carretero-Murillo, Mariana
AU - Kayed, Rakez
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2018.
PY - 2018
Y1 - 2018
N2 - An increasing number of studies have demonstrated the existence of multiple conformational entities of tau, as have been observed for prion protein. We have developed and optimized techniques to isolate and study oligomeric tau strains both in vitro and ex vivo. Moreover, we have modified protocols that demonstrate the seeding properties of oligomeric tau strains that are capable of propagating in vivo. These methods and protocols are explained in this chapter.
AB - An increasing number of studies have demonstrated the existence of multiple conformational entities of tau, as have been observed for prion protein. We have developed and optimized techniques to isolate and study oligomeric tau strains both in vitro and ex vivo. Moreover, we have modified protocols that demonstrate the seeding properties of oligomeric tau strains that are capable of propagating in vivo. These methods and protocols are explained in this chapter.
KW - In vitro tau seeding
KW - In vivo tau propagation
KW - Tau oligomer isolation
KW - Tau oligomeric strains
UR - http://www.scopus.com/inward/record.url?scp=85048511429&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048511429&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7816-8_9
DO - 10.1007/978-1-4939-7816-8_9
M3 - Chapter
AN - SCOPUS:85048511429
T3 - Methods in Molecular Biology
SP - 113
EP - 146
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -