Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

Carina Sihlbom; Pia Davidsson; Carol L. Nilsson

doi:10.1021/pr050210g

Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

Carina Sihlbom, Pia Davidsson, Carol L. Nilsson

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

Original language	English (US)
Pages (from-to)	2294-2301
Number of pages	8
Journal	Journal of Proteome Research
Volume	4
Issue number	6
DOIs	https://doi.org/10.1021/pr050210g
State	Published - Nov 2005
Externally published	Yes

Keywords

2-DE
Albumin removal
CSF
FT-ICR MS
Glycoprotein
Prefractionation

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr050210g

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AU - Nilsson, Carol L.

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AB - Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

KW - 2-DE

KW - Albumin removal

KW - CSF

KW - FT-ICR MS

KW - Glycoprotein

KW - Prefractionation

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Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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