Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

Lucy M. Ndip; Roland N. Ndip; Seraphine N. Esemu; David H. Walker; Jere W. McBride

doi:10.1007/s10493-009-9293-8

Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

Lucy M. Ndip, Roland N. Ndip, Seraphine N. Esemu, David H. Walker, Jere W. McBride

Pathology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.

Original language	English (US)
Pages (from-to)	163-168
Number of pages	6
Journal	Experimental and Applied Acarology
Volume	50
Issue number	2
DOIs	https://doi.org/10.1007/s10493-009-9293-8
State	Published - 2010

Keywords

Cameroon
Ehrlichia canis
Ehrlichia chaffeensis
Rhipicephalus sanguineus
Zoonosis

ASJC Scopus subject areas

Ecology
Insect Science

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title = "Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon",

abstract = "Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.",

keywords = "Cameroon, Ehrlichia canis, Ehrlichia chaffeensis, Rhipicephalus sanguineus, Zoonosis",

author = "Ndip, {Lucy M.} and Ndip, {Roland N.} and Esemu, {Seraphine N.} and Walker, {David H.} and McBride, {Jere W.}",

note = "Funding Information: Acknowledgments We thank C. Kuyler Doyle for assistance with real-time PCR and Alertia Tarkang for assistance with tick collection. This work was supported by the Forgarty International Center (grant D43TW00903 to D.H.W.) and the Sealy Center for Vaccine Development.",

year = "2010",

doi = "10.1007/s10493-009-9293-8",

language = "English (US)",

volume = "50",

pages = "163--168",

journal = "Experimental and Applied Acarology",

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T1 - Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

AU - Ndip, Lucy M.

AU - Ndip, Roland N.

AU - Esemu, Seraphine N.

AU - Walker, David H.

AU - McBride, Jere W.

N1 - Funding Information: Acknowledgments We thank C. Kuyler Doyle for assistance with real-time PCR and Alertia Tarkang for assistance with tick collection. This work was supported by the Forgarty International Center (grant D43TW00903 to D.H.W.) and the Sealy Center for Vaccine Development.

PY - 2010

Y1 - 2010

N2 - Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.

AB - Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.

KW - Cameroon

KW - Ehrlichia canis

KW - Ehrlichia chaffeensis

KW - Rhipicephalus sanguineus

KW - Zoonosis

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Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

Abstract

Keywords

ASJC Scopus subject areas

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