Porphyrin Accumulation and Biliary Lithiasis Causing Diffusely Black Livers in Broiler Chickens

Veronica Nguyen; Asli Mete; Anibal Armien; Ana P. Da Silva; Patrick Montine; Charles Corsiglia; V. M.Sadagopa Ramanujam; Karl E. Anderson; Ruediger Hauck; Rodrigo A. Gallardo

doi:10.1637/aviandiseases-D-21-00122

Porphyrin Accumulation and Biliary Lithiasis Causing Diffusely Black Livers in Broiler Chickens

Veronica Nguyen, Asli Mete, Anibal Armien, Ana P. Da Silva, Patrick Montine, Charles Corsiglia, V. M.Sadagopa Ramanujam, Karl E. Anderson, Ruediger Hauck, Rodrigo A. Gallardo

Internal Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

SUMMARY. Two 7-wk-old broiler chickens presented with uniformly black livers upon postslaughter examination, while all other organs as well as their carcasses were grossly normal. No clinical signs were reported by the field veterinarian prior to slaughter. Other broiler chickens within the same flock were unaffected. Microscopically, the liver exhibited variably sized, globoid concrements that were dark brown to green-brown and birefringent under polarized light. Ultrastructurally, concrements consisted of radially arranged electron-dense crystal spicules. Concrements were located in hepatocytes, within ecstatic bile canaliculi, or surrounded by small clusters of macrophages. Liquid chromatography assay determined the presence of protoporphyrin IX in the affected liver.

Original language	English (US)
Journal	Avian diseases
Volume	66
Issue number	2
DOIs	https://doi.org/10.1637/aviandiseases-D-21-00122
State	Published - Jun 2022

Keywords

Black liver
Broiler chickens
Electron microscopy
Pigment deposition
Porphyrin

ASJC Scopus subject areas

Food Animals
Animal Science and Zoology
General Immunology and Microbiology

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10.1637/aviandiseases-D-21-00122

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@article{75ef825b1e69435d815347c91960629d,

title = "Porphyrin Accumulation and Biliary Lithiasis Causing Diffusely Black Livers in Broiler Chickens",

abstract = "SUMMARY. Two 7-wk-old broiler chickens presented with uniformly black livers upon postslaughter examination, while all other organs as well as their carcasses were grossly normal. No clinical signs were reported by the field veterinarian prior to slaughter. Other broiler chickens within the same flock were unaffected. Microscopically, the liver exhibited variably sized, globoid concrements that were dark brown to green-brown and birefringent under polarized light. Ultrastructurally, concrements consisted of radially arranged electron-dense crystal spicules. Concrements were located in hepatocytes, within ecstatic bile canaliculi, or surrounded by small clusters of macrophages. Liquid chromatography assay determined the presence of protoporphyrin IX in the affected liver.",

keywords = "Black liver, Broiler chickens, Electron microscopy, Pigment deposition, Porphyrin",

author = "Veronica Nguyen and Asli Mete and Anibal Armien and {Da Silva}, {Ana P.} and Patrick Montine and Charles Corsiglia and Ramanujam, {V. M.Sadagopa} and Anderson, {Karl E.} and Ruediger Hauck and Gallardo, {Rodrigo A.}",

year = "2022",

month = jun,

doi = "10.1637/aviandiseases-D-21-00122",

language = "English (US)",

volume = "66",

journal = "Avian diseases",

issn = "0005-2086",

publisher = "American Association of Avian Pathologists",

number = "2",

}

TY - JOUR

T1 - Porphyrin Accumulation and Biliary Lithiasis Causing Diffusely Black Livers in Broiler Chickens

AU - Nguyen, Veronica

AU - Mete, Asli

AU - Armien, Anibal

AU - Da Silva, Ana P.

AU - Montine, Patrick

AU - Corsiglia, Charles

AU - Ramanujam, V. M.Sadagopa

AU - Anderson, Karl E.

AU - Hauck, Ruediger

AU - Gallardo, Rodrigo A.

PY - 2022/6

Y1 - 2022/6

N2 - SUMMARY. Two 7-wk-old broiler chickens presented with uniformly black livers upon postslaughter examination, while all other organs as well as their carcasses were grossly normal. No clinical signs were reported by the field veterinarian prior to slaughter. Other broiler chickens within the same flock were unaffected. Microscopically, the liver exhibited variably sized, globoid concrements that were dark brown to green-brown and birefringent under polarized light. Ultrastructurally, concrements consisted of radially arranged electron-dense crystal spicules. Concrements were located in hepatocytes, within ecstatic bile canaliculi, or surrounded by small clusters of macrophages. Liquid chromatography assay determined the presence of protoporphyrin IX in the affected liver.

AB - SUMMARY. Two 7-wk-old broiler chickens presented with uniformly black livers upon postslaughter examination, while all other organs as well as their carcasses were grossly normal. No clinical signs were reported by the field veterinarian prior to slaughter. Other broiler chickens within the same flock were unaffected. Microscopically, the liver exhibited variably sized, globoid concrements that were dark brown to green-brown and birefringent under polarized light. Ultrastructurally, concrements consisted of radially arranged electron-dense crystal spicules. Concrements were located in hepatocytes, within ecstatic bile canaliculi, or surrounded by small clusters of macrophages. Liquid chromatography assay determined the presence of protoporphyrin IX in the affected liver.

KW - Black liver

KW - Broiler chickens

KW - Electron microscopy

KW - Pigment deposition

KW - Porphyrin

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DO - 10.1637/aviandiseases-D-21-00122

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SN - 0005-2086

VL - 66

JO - Avian diseases

JF - Avian diseases

IS - 2

ER -

Porphyrin Accumulation and Biliary Lithiasis Causing Diffusely Black Livers in Broiler Chickens

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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