Population variation of West Nile virus confers a host-specific fitness benefit in mosquitoes

Kelly A. Fitzpatrick; Eleanor R. Deardorff; Kendra Pesko; Doug E. Brackney; Bo Zhang; Edward Bedrick; Pei Yong Shi; Gregory D. Ebel

doi:10.1016/j.virol.2010.04.029

Population variation of West Nile virus confers a host-specific fitness benefit in mosquitoes

Kelly A. Fitzpatrick, Eleanor R. Deardorff, Kendra Pesko, Doug E. Brackney, Bo Zhang, Edward Bedrick, Pei Yong Shi, Gregory D. Ebel

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

West Nile virus is similar to most other RNA viruses in that it exists in nature as a genetically diverse population. However, the role of this genetic diversity within natural transmission cycles and its importance to virus perpetuation remain poorly understood. Therefore, we determined whether highly genetically diverse populations are more fit compared to less genetically diverse WNV populations. Specifically, we generated three WNV populations that varied in their genetic diversity and evaluated their fitness relative to genetically marked control WNV in vivo in Culex quinquefasciatus mosquitoes and chickens. Our results demonstrate that high genetic diversity leads to fitness gains in vector mosquitoes, but not chickens.

Original language	English (US)
Pages (from-to)	89-95
Number of pages	7
Journal	Virology
Volume	404
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2010.04.029
State	Published - Aug 2010
Externally published	Yes

Keywords

Arbovirus
Fitness
Flavivirus
Mosquitoes
Quasispecies
West Nile virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2010.04.029

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title = "Population variation of West Nile virus confers a host-specific fitness benefit in mosquitoes",

abstract = "West Nile virus is similar to most other RNA viruses in that it exists in nature as a genetically diverse population. However, the role of this genetic diversity within natural transmission cycles and its importance to virus perpetuation remain poorly understood. Therefore, we determined whether highly genetically diverse populations are more fit compared to less genetically diverse WNV populations. Specifically, we generated three WNV populations that varied in their genetic diversity and evaluated their fitness relative to genetically marked control WNV in vivo in Culex quinquefasciatus mosquitoes and chickens. Our results demonstrate that high genetic diversity leads to fitness gains in vector mosquitoes, but not chickens.",

keywords = "Arbovirus, Fitness, Flavivirus, Mosquitoes, Quasispecies, West Nile virus",

author = "Fitzpatrick, {Kelly A.} and Deardorff, {Eleanor R.} and Kendra Pesko and Brackney, {Doug E.} and Bo Zhang and Edward Bedrick and Shi, {Pei Yong} and Ebel, {Gregory D.}",

note = "Funding Information: The authors thank Damon Little for generous assistance in implementing PolySNP, Laura Kramer for helpful comments on the manuscript, and Ivy K. Brown and Robert Nofchissey for valuable technical assistance. Animal experiments were approved by the University of New Mexico Institutional Animal Care and Use Committee. Sequencing was performed at the UNM DNA Research Facility. This work was supported in part by funds from the National Institute of Allergy and Infectious Disease, National Institute of Health , under grant AI067380 . ",

year = "2010",

month = aug,

doi = "10.1016/j.virol.2010.04.029",

language = "English (US)",

volume = "404",

pages = "89--95",

journal = "Virology",

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AU - Fitzpatrick, Kelly A.

AU - Deardorff, Eleanor R.

AU - Pesko, Kendra

AU - Brackney, Doug E.

AU - Zhang, Bo

AU - Bedrick, Edward

AU - Shi, Pei Yong

AU - Ebel, Gregory D.

N1 - Funding Information: The authors thank Damon Little for generous assistance in implementing PolySNP, Laura Kramer for helpful comments on the manuscript, and Ivy K. Brown and Robert Nofchissey for valuable technical assistance. Animal experiments were approved by the University of New Mexico Institutional Animal Care and Use Committee. Sequencing was performed at the UNM DNA Research Facility. This work was supported in part by funds from the National Institute of Allergy and Infectious Disease, National Institute of Health , under grant AI067380 .

PY - 2010/8

Y1 - 2010/8

N2 - West Nile virus is similar to most other RNA viruses in that it exists in nature as a genetically diverse population. However, the role of this genetic diversity within natural transmission cycles and its importance to virus perpetuation remain poorly understood. Therefore, we determined whether highly genetically diverse populations are more fit compared to less genetically diverse WNV populations. Specifically, we generated three WNV populations that varied in their genetic diversity and evaluated their fitness relative to genetically marked control WNV in vivo in Culex quinquefasciatus mosquitoes and chickens. Our results demonstrate that high genetic diversity leads to fitness gains in vector mosquitoes, but not chickens.

AB - West Nile virus is similar to most other RNA viruses in that it exists in nature as a genetically diverse population. However, the role of this genetic diversity within natural transmission cycles and its importance to virus perpetuation remain poorly understood. Therefore, we determined whether highly genetically diverse populations are more fit compared to less genetically diverse WNV populations. Specifically, we generated three WNV populations that varied in their genetic diversity and evaluated their fitness relative to genetically marked control WNV in vivo in Culex quinquefasciatus mosquitoes and chickens. Our results demonstrate that high genetic diversity leads to fitness gains in vector mosquitoes, but not chickens.

KW - Arbovirus

KW - Fitness

KW - Flavivirus

KW - Mosquitoes

KW - Quasispecies

KW - West Nile virus

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Population variation of West Nile virus confers a host-specific fitness benefit in mosquitoes

Abstract

Keywords

ASJC Scopus subject areas

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