Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens

V. A. Feodorova; L. N. Pan'kina; E. P. Savostina; O. S. Kuznetsov; N. P. Konnov; L. V. Sayapina; S. V. Dentovskaya; R. Z. Shaikhutdinova; S. A. Ageev; B. Lindner; A. N. Kondakova; O. V. Bystrova; N. A. Kocharova; S. N. Senchenkova; O. Holst; G. B. Pier; Y. A. Knirel; A. P. Anisimov; V. L. Motin

doi:10.1016/j.vaccine.2009.02.020

Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens

V. A. Feodorova, L. N. Pan'kina, E. P. Savostina, O. S. Kuznetsov, N. P. Konnov, L. V. Sayapina, S. V. Dentovskaya, R. Z. Shaikhutdinova, S. A. Ageev, B. Lindner, A. N. Kondakova, O. V. Bystrova, N. A. Kocharova, S. N. Senchenkova, O. Holst, G. B. Pier, Y. A. Knirel, A. P. Anisimov, V. L. Motin

Pathology

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19 Scopus citations

Abstract

Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and immunoreactivity of numerous major proteins and carbohydrate antigens, including F1, Pla, Ymt, V antigen, LPS, and ECA. Moreover, both mutants demonstrated altered epitope specificities of the antigens as determined in immunodot-ELISAs and immunoblotting analyses using a panel of monoclonal antibodies. The strains also differed in their susceptibility to the diagnostic plague bacteriophage L-413C. These findings indicate that the effects of the lpxM mutation on reduced virulence and enhanced immunity of the Y. pestis EV ΔlpxM is also associated with these pleiotropic changes and not just to changes in the lipid A acylation.

Original language	English (US)
Pages (from-to)	2240-2250
Number of pages	11
Journal	Vaccine
Volume	27
Issue number	16
DOIs	https://doi.org/10.1016/j.vaccine.2009.02.020
State	Published - Apr 6 2009

Keywords

Live plague vaccine
lpxM(msbB) mutant
Plague immunity
Virulence
Yersinia pestis 231
Yersinia pestis antigens
Yersinia pestis EV NIIEG

ASJC Scopus subject areas

General Immunology and Microbiology
Infectious Diseases
Public Health, Environmental and Occupational Health
General Veterinary
Molecular Medicine

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10.1016/j.vaccine.2009.02.020

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Feodorova, V. A., Pan'kina, L. N., Savostina, E. P., Kuznetsov, O. S., Konnov, N. P., Sayapina, L. V., Dentovskaya, S. V., Shaikhutdinova, R. Z., Ageev, S. A., Lindner, B., Kondakova, A. N., Bystrova, O. V., Kocharova, N. A., Senchenkova, S. N., Holst, O., Pier, G. B., Knirel, Y. A., Anisimov, A. P., & Motin, V. L. (2009). Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens. Vaccine, 27(16), 2240-2250. https://doi.org/10.1016/j.vaccine.2009.02.020

Feodorova, VA, Pan'kina, LN, Savostina, EP, Kuznetsov, OS, Konnov, NP, Sayapina, LV, Dentovskaya, SV, Shaikhutdinova, RZ, Ageev, SA, Lindner, B, Kondakova, AN, Bystrova, OV, Kocharova, NA, Senchenkova, SN, Holst, O, Pier, GB, Knirel, YA, Anisimov, AP & Motin, VL 2009, 'Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens', Vaccine, vol. 27, no. 16, pp. 2240-2250. https://doi.org/10.1016/j.vaccine.2009.02.020

@article{cd32620683d24cefb9ee83f0660c4ad3,

title = "Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens",

abstract = "Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and immunoreactivity of numerous major proteins and carbohydrate antigens, including F1, Pla, Ymt, V antigen, LPS, and ECA. Moreover, both mutants demonstrated altered epitope specificities of the antigens as determined in immunodot-ELISAs and immunoblotting analyses using a panel of monoclonal antibodies. The strains also differed in their susceptibility to the diagnostic plague bacteriophage L-413C. These findings indicate that the effects of the lpxM mutation on reduced virulence and enhanced immunity of the Y. pestis EV ΔlpxM is also associated with these pleiotropic changes and not just to changes in the lipid A acylation.",

keywords = "Live plague vaccine, lpxM(msbB) mutant, Plague immunity, Virulence, Yersinia pestis 231, Yersinia pestis antigens, Yersinia pestis EV NIIEG",

author = "Feodorova, {V. A.} and Pan'kina, {L. N.} and Savostina, {E. P.} and Kuznetsov, {O. S.} and Konnov, {N. P.} and Sayapina, {L. V.} and Dentovskaya, {S. V.} and Shaikhutdinova, {R. Z.} and Ageev, {S. A.} and B. Lindner and Kondakova, {A. N.} and Bystrova, {O. V.} and Kocharova, {N. A.} and Senchenkova, {S. N.} and O. Holst and Pier, {G. B.} and Knirel, {Y. A.} and Anisimov, {A. P.} and Motin, {V. L.}",

note = "Funding Information: This work was supported by the Russian Foundation for Basic Research (grants 06-04-49280 and 03-04-48067), the Western RCE, NIH (award 1-U54-AI-057156), the Council on Grants at the President of the Russian Federation for Support of Young Russian Scientists (grant MK-5304.2007.4), the German Research Foundation (grant LI-448-1), the US National Institutes of Health (grant AI46706-06) and the International Science and Technology Center (ISTC) (grants #3730 and #3853). ",

year = "2009",

month = apr,

day = "6",

doi = "10.1016/j.vaccine.2009.02.020",

language = "English (US)",

volume = "27",

pages = "2240--2250",

journal = "Vaccine",

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publisher = "Elsevier BV",

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T1 - Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens

AU - Feodorova, V. A.

AU - Pan'kina, L. N.

AU - Savostina, E. P.

AU - Kuznetsov, O. S.

AU - Konnov, N. P.

AU - Sayapina, L. V.

AU - Dentovskaya, S. V.

AU - Shaikhutdinova, R. Z.

AU - Ageev, S. A.

AU - Lindner, B.

AU - Kondakova, A. N.

AU - Bystrova, O. V.

AU - Kocharova, N. A.

AU - Senchenkova, S. N.

AU - Holst, O.

AU - Pier, G. B.

AU - Knirel, Y. A.

AU - Anisimov, A. P.

AU - Motin, V. L.

N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (grants 06-04-49280 and 03-04-48067), the Western RCE, NIH (award 1-U54-AI-057156), the Council on Grants at the President of the Russian Federation for Support of Young Russian Scientists (grant MK-5304.2007.4), the German Research Foundation (grant LI-448-1), the US National Institutes of Health (grant AI46706-06) and the International Science and Technology Center (ISTC) (grants #3730 and #3853).

PY - 2009/4/6

Y1 - 2009/4/6

N2 - Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and immunoreactivity of numerous major proteins and carbohydrate antigens, including F1, Pla, Ymt, V antigen, LPS, and ECA. Moreover, both mutants demonstrated altered epitope specificities of the antigens as determined in immunodot-ELISAs and immunoblotting analyses using a panel of monoclonal antibodies. The strains also differed in their susceptibility to the diagnostic plague bacteriophage L-413C. These findings indicate that the effects of the lpxM mutation on reduced virulence and enhanced immunity of the Y. pestis EV ΔlpxM is also associated with these pleiotropic changes and not just to changes in the lipid A acylation.

AB - Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and immunoreactivity of numerous major proteins and carbohydrate antigens, including F1, Pla, Ymt, V antigen, LPS, and ECA. Moreover, both mutants demonstrated altered epitope specificities of the antigens as determined in immunodot-ELISAs and immunoblotting analyses using a panel of monoclonal antibodies. The strains also differed in their susceptibility to the diagnostic plague bacteriophage L-413C. These findings indicate that the effects of the lpxM mutation on reduced virulence and enhanced immunity of the Y. pestis EV ΔlpxM is also associated with these pleiotropic changes and not just to changes in the lipid A acylation.

KW - Live plague vaccine

KW - lpxM(msbB) mutant

KW - Plague immunity

KW - Virulence

KW - Yersinia pestis 231

KW - Yersinia pestis antigens

KW - Yersinia pestis EV NIIEG

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U2 - 10.1016/j.vaccine.2009.02.020

DO - 10.1016/j.vaccine.2009.02.020

M3 - Article

C2 - 19428838

AN - SCOPUS:62149141733

SN - 0264-410X

VL - 27

SP - 2240

EP - 2250

JO - Vaccine

JF - Vaccine

IS - 16

ER -

Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens

Abstract

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