TY - JOUR
T1 - Pingu virus
T2 - A new picornavirus in penguins from Antarctica
AU - De Souza, William Marciel
AU - Fumagalli, Marcílio Jorge
AU - Martin, Matheus Cavalheiro
AU - De Araujo, Jansen
AU - Orsi, Maria Angela
AU - Sanfilippo, Luiz Francisco
AU - Modha, Sejal
AU - Durigon, Edison Luiz
AU - Proença-Módena, José Luiz
AU - Arns, Clarice Weis
AU - Murcia, Pablo Ramiro
AU - Figueiredo, Luiz Tadeu Moraes
N1 - Publisher Copyright:
© 2019 The Author(s).
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Picornaviridae family comprises single-stranded, positive-sense RNA viruses distributed into forty-seven genera. Picornaviruses have a broad host range and geographic distribution in all continents. In this study, we applied a high-throughput sequencing approach to examine the presence of picornaviruses in penguins from King George Island, Antarctica. We discovered and characterized a novel picornavirus from cloacal swab samples of gentoo penguins (Pygoscelis papua), which we tentatively named Pingu virus. Also, using RT-PCR we detected this virus in 12.9 per cent of cloacal swabs derived from P. papua, but not in samples from adélie penguins (Pygoscelis adeliae) or chinstrap penguins (Pygoscelis antarcticus). Attempts to isolate the virus in a chicken cell line and in embryonated chicken eggs were unsuccessful. Our results expand the viral diversity, host range, and geographical distribution of the Picornaviridae.
AB - Picornaviridae family comprises single-stranded, positive-sense RNA viruses distributed into forty-seven genera. Picornaviruses have a broad host range and geographic distribution in all continents. In this study, we applied a high-throughput sequencing approach to examine the presence of picornaviruses in penguins from King George Island, Antarctica. We discovered and characterized a novel picornavirus from cloacal swab samples of gentoo penguins (Pygoscelis papua), which we tentatively named Pingu virus. Also, using RT-PCR we detected this virus in 12.9 per cent of cloacal swabs derived from P. papua, but not in samples from adélie penguins (Pygoscelis adeliae) or chinstrap penguins (Pygoscelis antarcticus). Attempts to isolate the virus in a chicken cell line and in embryonated chicken eggs were unsuccessful. Our results expand the viral diversity, host range, and geographical distribution of the Picornaviridae.
KW - Antarctica
KW - metagenomics
KW - penguins
KW - picornavirus
KW - virus discovery
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U2 - 10.1093/ve/vez047
DO - 10.1093/ve/vez047
M3 - Article
AN - SCOPUS:85096664835
SN - 2057-1577
VL - 5
JO - Virus Evolution
JF - Virus Evolution
IS - 2
M1 - vez047
ER -