Persistent infection with mouse hepatitis virus, JHM strain in DBT cell culture.

N. Hirano, N. Goto, S. Makino, K. Fujiwara

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


After inoculation with JHM strain into DBT cell monolayers, a persistently infected DBT cell culture was established without producing typical cytopathic changes after about 15th passages. By immunofluorescence virus specific antigen was demonstrated in 10 to 15% DBT cells. This persistently infected culture (JHM-CC) was resistant to superinfection with parental JHM, but such resistance was not shown against vesicular stomatitis virus. JHM-CC virus produced small plaques on DBT cell monolayers. Temperature sensitive (TS) mutant, defective interfering (DI) particle or interferon was not detected in the JHM-CC. To intracerebral inoculation with JHM-CC virus, cortisone treated ICR mice survived without showing clinical signs, however, demyelinating lesions were produced in the brain and spinal cord of them.

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalAdvances in experimental medicine and biology
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


Dive into the research topics of 'Persistent infection with mouse hepatitis virus, JHM strain in DBT cell culture.'. Together they form a unique fingerprint.

Cite this