Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties

C. J. Orihuela, R. Janssen, C. W. Robb, D. A. Watson, D. W. Niesel

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.

Original languageEnglish (US)
Pages (from-to)6082-6086
Number of pages5
JournalInfection and immunity
Issue number10
StatePublished - 2000

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


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