Pathogenicity Comparison between the Kikwit and Makona Ebola Virus Variants in Rhesus Macaques

Gary Wong; Xiangguo Qiu; Marc Antoine De La Vega; Lisa Fernando; Haiyan Wei; Alexander Bello; Hugues Fausther-Bovendo; Jonathan Audet; Andrea Kroeker; Robert Kozak; Kaylie Tran; Shihua He; Kevin Tierney; Geoff Soule; Estella Moffat; Stephan Günther; George F. Gao; Jim Strong; Carissa Embury-Hyatt; Gary Kobinger

doi:10.1093/infdis/jiw267

Pathogenicity Comparison between the Kikwit and Makona Ebola Virus Variants in Rhesus Macaques

Gary Wong, Xiangguo Qiu, Marc Antoine De La Vega, Lisa Fernando, Haiyan Wei, Alexander Bello, Hugues Fausther-Bovendo, Jonathan Audet, Andrea Kroeker, Robert Kozak, Kaylie Tran, Shihua He, Kevin Tierney, Geoff Soule, Estella Moffat, Stephan Günther, George F. Gao, Jim Strong, Carissa Embury-Hyatt, Gary Kobinger

Research output: Contribution to journal › Article › peer-review

Abstract

Enhanced virulence and/or transmission of West African Ebola virus (EBOV) variants, which are divergent from their Central African counterparts, are suspected to have contributed to the sizable toll of the recent Ebola virus disease (EVD) outbreak. This study evaluated the pathogenicity and shedding in rhesus macaques infected with 1 of 2 West African isolates (EBOV-C05 or EBOV-C07) or a Central African isolate (EBOV-K). All animals infected with EBOV-C05 or EBOV-C07 died of EVD, whereas 2 of 3 EBOV-K-infected animals died. The viremia level was elevated 10-fold in EBOV-C05-infected animals, compared with EBOV-C07- or EBOV-K-infected animals. More-severe lung pathology was observed in 2 of 6 EBOV-C05/C07-infected macaques. This is the first detailed analysis of the recently circulating EBOV-C05/C07 in direct comparison to EBOV-K with 6 animals per group, and it showed that EBOV-C05 but not EBOV-C07 can replicate at higher levels and cause more tissue damage in some animals. Increased virus shedding from individuals who are especially susceptible to EBOV replication is possibly one of the many challenges facing the community of healthcare and policy-making responders since the beginning of the outbreak.

Original language	English (US)
Pages (from-to)	S281-S289
Journal	Journal of Infectious Diseases
Volume	214
DOIs	https://doi.org/10.1093/infdis/jiw267
State	Published - Oct 15 2016
Externally published	Yes

Keywords

Ebola virus
Kikwit
Makona
Nonhuman primates
Pathogenicity

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1093/infdis/jiw267

Cite this

Wong, G., Qiu, X., De La Vega, M. A., Fernando, L., Wei, H., Bello, A., Fausther-Bovendo, H., Audet, J., Kroeker, A., Kozak, R., Tran, K., He, S., Tierney, K., Soule, G., Moffat, E., Günther, S., Gao, G. F., Strong, J., Embury-Hyatt, C., & Kobinger, G. (2016). Pathogenicity Comparison between the Kikwit and Makona Ebola Virus Variants in Rhesus Macaques. Journal of Infectious Diseases, 214, S281-S289. https://doi.org/10.1093/infdis/jiw267

Wong, G, Qiu, X, De La Vega, MA, Fernando, L, Wei, H, Bello, A, Fausther-Bovendo, H, Audet, J, Kroeker, A, Kozak, R, Tran, K, He, S, Tierney, K, Soule, G, Moffat, E, Günther, S, Gao, GF, Strong, J, Embury-Hyatt, C & Kobinger, G 2016, 'Pathogenicity Comparison between the Kikwit and Makona Ebola Virus Variants in Rhesus Macaques', Journal of Infectious Diseases, vol. 214, pp. S281-S289. https://doi.org/10.1093/infdis/jiw267

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abstract = "Enhanced virulence and/or transmission of West African Ebola virus (EBOV) variants, which are divergent from their Central African counterparts, are suspected to have contributed to the sizable toll of the recent Ebola virus disease (EVD) outbreak. This study evaluated the pathogenicity and shedding in rhesus macaques infected with 1 of 2 West African isolates (EBOV-C05 or EBOV-C07) or a Central African isolate (EBOV-K). All animals infected with EBOV-C05 or EBOV-C07 died of EVD, whereas 2 of 3 EBOV-K-infected animals died. The viremia level was elevated 10-fold in EBOV-C05-infected animals, compared with EBOV-C07- or EBOV-K-infected animals. More-severe lung pathology was observed in 2 of 6 EBOV-C05/C07-infected macaques. This is the first detailed analysis of the recently circulating EBOV-C05/C07 in direct comparison to EBOV-K with 6 animals per group, and it showed that EBOV-C05 but not EBOV-C07 can replicate at higher levels and cause more tissue damage in some animals. Increased virus shedding from individuals who are especially susceptible to EBOV replication is possibly one of the many challenges facing the community of healthcare and policy-making responders since the beginning of the outbreak.",

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doi = "10.1093/infdis/jiw267",

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AU - Qiu, Xiangguo

AU - De La Vega, Marc Antoine

AU - Fernando, Lisa

AU - Wei, Haiyan

AU - Bello, Alexander

AU - Fausther-Bovendo, Hugues

AU - Audet, Jonathan

AU - Kroeker, Andrea

AU - Kozak, Robert

AU - Tran, Kaylie

AU - He, Shihua

AU - Tierney, Kevin

AU - Soule, Geoff

AU - Moffat, Estella

AU - Günther, Stephan

AU - Gao, George F.

AU - Strong, Jim

AU - Embury-Hyatt, Carissa

AU - Kobinger, Gary

PY - 2016/10/15

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KW - Makona

KW - Nonhuman primates

KW - Pathogenicity

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JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

ER -

Pathogenicity Comparison between the Kikwit and Makona Ebola Virus Variants in Rhesus Macaques

Abstract

Keywords

ASJC Scopus subject areas

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