Partial genome molecular characteristics of Getah virus newly isolated in China

Wei Xin Chen, Huan Yu Wang, Shi Hong Fu, Ming Hua Li, Gui Fang Liu, Hong Yue Jiang, Li Hua Wang, Hai Yan Wang, Zhi Yu Wang, Guo Dong Liang

Research output: Contribution to journalArticlepeer-review


Objective: To study the genome molecular characteristics of Getah virus (DY0824) which isolated in Shandong province, 2008 by molecular biology methods. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify the structural gene and 3'UTR fragments then the RT-PCR products were inserted into PGEM-T easy to be sequenced. Computer software was used to analyze the nucleotide and deduced amino acid sequence, and draw phylogenetic trees, including Clustal X1.83 and MegaAlign and Mega4. Results: The capsid protein of DY0824 consists of 804 nucleotides, encoding 268 amino acids and the full-length of E2 protein is 1266 nucleotides, encoding 422 amino acids. The nucleotide homology of the capsid protein and the E2 protein with other strains were 95.4%-99.9% and 94.8% -99.5%, and the amino acid were 97.4%-100% and 97.6%-100%. The 3'UTR of the virus include 401 nucleotides and there are three repeat sequence elements. Conclusion: Compared with the prototype virus, the Getah virus isolated in Shandong province had 7 amino acid differences in capsid protein genes and 10 amino acid differences in E protein genes. The 3'UTR region had multi-nucleotide changes.

Original languageEnglish (US)
Pages (from-to)399-404
Number of pages6
JournalChinese Journal of Microbiology and Immunology
Issue number5
StatePublished - 2010
Externally publishedYes


  • 3' untranslated region
  • Capsid gene
  • E2 protein gene
  • Getah virus
  • Nucleotide sequence

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology


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