Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases

Gustavo Palacios; Phenix Lan Quan; Omar J. Jabado; Sean Conlan; David L. Hirschberg; Yang Liu; Junhui Zhai; Neil Renwick; Jeffrey Hui; Hedi Hegyi; Allen Grolla; James E. Strong; Jonathan S. Towner; Thomas W. Geisbert; Peter B. Jahrling; Cornelia Büchen-Osmond; Heinz Ellerbrok; Maria Paz Sanchez-Seco; Yves Lussier; Pierre Formenty; Stuart T. Nichol; Heinz Feldmann; Thomas Briese; W. Ian Lipkin

doi:10.3201/eid1301.060837

Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases

Gustavo Palacios, Phenix Lan Quan, Omar J. Jabado, Sean Conlan, David L. Hirschberg, Yang Liu, Junhui Zhai, Neil Renwick, Jeffrey Hui, Hedi Hegyi, Allen Grolla, James E. Strong, Jonathan S. Towner, Thomas W. Geisbert, Peter B. Jahrling, Cornelia Büchen-Osmond, Heinz Ellerbrok, Maria Paz Sanchez-Seco, Yves Lussier, Pierre FormentyStuart T. Nichol, Heinz Feldmann, Thomas Briese, W. Ian Lipkin

Research output: Contribution to journal › Article › peer-review

229 Scopus citations

Abstract

To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.

Original language	English (US)
Pages (from-to)	73-81
Number of pages	9
Journal	Emerging infectious diseases
Volume	13
Issue number	1
DOIs	https://doi.org/10.3201/eid1301.060837
State	Published - Jan 2007
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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Palacios, G., Quan, P. L., Jabado, O. J., Conlan, S., Hirschberg, D. L., Liu, Y., Zhai, J., Renwick, N., Hui, J., Hegyi, H., Grolla, A., Strong, J. E., Towner, J. S., Geisbert, T. W., Jahrling, P. B., Büchen-Osmond, C., Ellerbrok, H., Sanchez-Seco, M. P., Lussier, Y., ... Lipkin, W. I. (2007). Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases. Emerging infectious diseases, 13(1), 73-81. https://doi.org/10.3201/eid1301.060837

Palacios, G, Quan, PL, Jabado, OJ, Conlan, S, Hirschberg, DL, Liu, Y, Zhai, J, Renwick, N, Hui, J, Hegyi, H, Grolla, A, Strong, JE, Towner, JS, Geisbert, TW, Jahrling, PB, Büchen-Osmond, C, Ellerbrok, H, Sanchez-Seco, MP, Lussier, Y, Formenty, P, Nichol, ST, Feldmann, H, Briese, T & Lipkin, WI 2007, 'Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases', Emerging infectious diseases, vol. 13, no. 1, pp. 73-81. https://doi.org/10.3201/eid1301.060837

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abstract = "To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.",

author = "Gustavo Palacios and Quan, {Phenix Lan} and Jabado, {Omar J.} and Sean Conlan and Hirschberg, {David L.} and Yang Liu and Junhui Zhai and Neil Renwick and Jeffrey Hui and Hedi Hegyi and Allen Grolla and Strong, {James E.} and Towner, {Jonathan S.} and Geisbert, {Thomas W.} and Jahrling, {Peter B.} and Cornelia B{\"u}chen-Osmond and Heinz Ellerbrok and Sanchez-Seco, {Maria Paz} and Yves Lussier and Pierre Formenty and Nichol, {Stuart T.} and Heinz Feldmann and Thomas Briese and Lipkin, {W. Ian}",

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AU - Jabado, Omar J.

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AU - Liu, Yang

AU - Zhai, Junhui

AU - Renwick, Neil

AU - Hui, Jeffrey

AU - Hegyi, Hedi

AU - Grolla, Allen

AU - Strong, James E.

AU - Towner, Jonathan S.

AU - Geisbert, Thomas W.

AU - Jahrling, Peter B.

AU - Büchen-Osmond, Cornelia

AU - Ellerbrok, Heinz

AU - Sanchez-Seco, Maria Paz

AU - Lussier, Yves

AU - Formenty, Pierre

AU - Nichol, Stuart T.

AU - Feldmann, Heinz

AU - Briese, Thomas

AU - Lipkin, W. Ian

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Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases

Abstract

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