Oxidation and structural perturbation of redox-sensitive enzymes in injured skeletal muscle

Anson P. Pierce, Eric de Waal, Linda M. McManus, Paula K. Shireman, Asish R. Chaudhuri

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Molecular events that control skeletal muscle injury and regeneration are poorly understood. However, inflammation associated with oxidative stress is considered a key player in modulating this process. To understand the consequences of oxidative stress associated with muscle injury, inflammation, and regeneration, hind-limb muscles of C57Bl/6J mice were studied after injection of cardiotoxin (CT). Within 1 day post-CT injection, polymorphonuclear neutrophilic leukocyte accumulation was extensive. Compared to baseline, tissue myeloperoxidase (MPO) activity was elevated eight- and fivefold at 1 and 7 days post-CT, respectively. Ubiquitinylated protein was elevated 1 day postinjury and returned to baseline by 21 days. Cysteine residues of creatine kinase (CK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were irreversibly oxidized within 1 day post-CT injection and were associated with protein conformational changes that fully recovered after 21 days. Importantly, protein structural alterations occurred in conjunction with significant decreases in CK activity at 1, 3, and 7 days post-CT injury. Interestingly, elevations in tissue MPO activity paralleled the time course of conformational changes in CK and GAPDH. In combination, these results demonstrate that muscle proteins in vivo are structurally and functionally altered via the generation of reactive oxygen species produced during inflammatory events after muscle injury and preceding muscle regeneration.

Original languageEnglish (US)
Pages (from-to)1584-1593
Number of pages10
JournalFree Radical Biology and Medicine
Issue number12
StatePublished - Dec 15 2007
Externally publishedYes


  • BisANS
  • Cardiotoxin
  • Creatine kinase
  • Free radicals
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Skeletal muscle injury

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


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