Optical biosensing of markers of mucosal inflammation

Obdulia Covarrubias-Zambrano; Massoud Motamedi; Bill T. Ameredes; Bing Tian; William J. Calhoun; Yingxin Zhao; Allan R. Brasier; Madumali Kalubowilage; Aruni P. Malalasekera; Asanka S. Yapa; Hongwang Wang; Christopher T. Culbertson; Deryl L. Troyer; Stefan H. Bossmann

doi:10.1016/j.nano.2021.102476

Optical biosensing of markers of mucosal inflammation

Obdulia Covarrubias-Zambrano, Massoud Motamedi, Bill T. Ameredes, Bing Tian, William J. Calhoun, Yingxin Zhao, Allan R. Brasier, Madumali Kalubowilage, Aruni P. Malalasekera, Asanka S. Yapa, Hongwang Wang, Christopher T. Culbertson, Deryl L. Troyer, Stefan H. Bossmann

Research output: Contribution to journal › Article › peer-review

Abstract

We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.

Original language	English (US)
Article number	102476
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	40
DOIs	https://doi.org/10.1016/j.nano.2021.102476
State	Published - Feb 2022

Keywords

Iron/iron oxide core/shell nanoparticle
Lung inflammation
Nanodiagnostics
Nanomedicine
Optical biosensor

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
General Materials Science
Pharmaceutical Science

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10.1016/j.nano.2021.102476

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Covarrubias-Zambrano, O., Motamedi, M., Ameredes, B. T., Tian, B., Calhoun, W. J., Zhao, Y., Brasier, A. R., Kalubowilage, M., Malalasekera, A. P., Yapa, A. S., Wang, H., Culbertson, C. T., Troyer, D. L., & Bossmann, S. H. (2022). Optical biosensing of markers of mucosal inflammation. Nanomedicine: Nanotechnology, Biology, and Medicine, 40, Article 102476. https://doi.org/10.1016/j.nano.2021.102476

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abstract = "We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.",

keywords = "Iron/iron oxide core/shell nanoparticle, Lung inflammation, Nanodiagnostics, Nanomedicine, Optical biosensor",

author = "Obdulia Covarrubias-Zambrano and Massoud Motamedi and Ameredes, {Bill T.} and Bing Tian and Calhoun, {William J.} and Yingxin Zhao and Brasier, {Allan R.} and Madumali Kalubowilage and Malalasekera, {Aruni P.} and Yapa, {Asanka S.} and Hongwang Wang and Culbertson, {Christopher T.} and Troyer, {Deryl L.} and Bossmann, {Stefan H.}",

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year = "2022",

month = feb,

doi = "10.1016/j.nano.2021.102476",

language = "English (US)",

volume = "40",

journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",

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AU - Covarrubias-Zambrano, Obdulia

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AU - Ameredes, Bill T.

AU - Tian, Bing

AU - Calhoun, William J.

AU - Zhao, Yingxin

AU - Brasier, Allan R.

AU - Kalubowilage, Madumali

AU - Malalasekera, Aruni P.

AU - Yapa, Asanka S.

AU - Wang, Hongwang

AU - Culbertson, Christopher T.

AU - Troyer, Deryl L.

AU - Bossmann, Stefan H.

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N2 - We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.

AB - We report the design and adaptation of iron/iron oxide nanoparticle-based optical nanobiosensors for enzymes or cytokine/chemokines that are established biomarkers of lung diseases. These biomarkers comprise ADAM33, granzyme B, MMP-8, neutrophil elastase, arginase, chemokine (C-C motif) ligand 20 and interleukin-6. The synthesis of nanobiosensors for these seven biomarkers, their calibration with commercially available enzymes and cytokines/chemokines, as well as their validation using bronchoalveolar lavage (BAL) obtained from a mouse model of TLR3-mediated inflammation are discussed here. Exhaled Breath Condensate (EBC) is a minimally invasive approach for sampling airway fluid in the diagnosis and management of various lung diseases in humans (e.g., asthma, COPD and viral infections). We report the proof-of-concept of using human EBC in conjunction with nanobiosensors for diagnosis/monitoring airway inflammation. These findings suggest that, with nanosensor technology, human EBC can be utilized as a liquid biopsy to monitor inflammation/remodeling in lung disease.

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Optical biosensing of markers of mucosal inflammation

Abstract

Keywords

ASJC Scopus subject areas

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