Opposing Effects of PKCθ and WASp on Symmetry Breaking and Relocation of the Immunological Synapse

Tasha N. Sims, Timothy J. Soos, Harry S. Xenias, Benjamin Dubin-Thaler, Jake M. Hofman, Janelle C. Waite, Thomas O. Cameron, V. Kaye Thomas, Rajat Varma, Chris H. Wiggins, Michael P. Sheetz, Dan R. Littman, Michael L. Dustin

Research output: Contribution to journalArticlepeer-review

256 Scopus citations


The immunological synapse (IS) is a junction between the T cell and antigen-presenting cell and is composed of supramolecular activation clusters (SMACs). No studies have been published on naive T cell IS dynamics. Here, we find that IS formation during antigen recognition comprises cycles of stable IS formation and autonomous naive T cell migration. The migration phase is driven by PKCθ, which is localized to the F-actin-dependent peripheral (p)SMAC. PKCθ-/- T cells formed hyperstable IS in vitro and in vivo and, like WT cells, displayed fast oscillations in the distal SMAC, but they showed reduced slow oscillations in pSMAC integrity. IS reformation is driven by the Wiscott Aldrich Syndrome protein (WASp). WASp-/- T cells displayed normal IS formation but were unable to reform IS after migration unless PKCθ was inhibited. Thus, opposing effects of PKCθ and WASp control IS stability through pSMAC symmetry breaking and reformation.

Original languageEnglish (US)
Pages (from-to)773-785
Number of pages13
Issue number4
StatePublished - May 18 2007
Externally publishedYes



ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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