On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes

Lena Lewin; Mats Olof Mattsson; David K. Rassin; Åke Sellström

doi:10.1007/BF00974574

On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes

Lena Lewin, Mats Olof Mattsson, David K. Rassin, Åke Sellström

Pediatrics

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The uptake of radioactive γ-aminobutyric acid (GABA) and d-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P₂-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [³H]GABA and for d-[³H]aspartate with an increasing V_max during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P₂-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake of d-aspartate. We found no non-saturable uptake of d-aspartate.

Original language	English (US)
Pages (from-to)	333-337
Number of pages	5
Journal	Neurochemical Research
Volume	17
Issue number	4
DOIs	https://doi.org/10.1007/BF00974574
State	Published - Apr 1992

Keywords

GABA
SKF 89976-A
d-aspartate
glutamate
primary neuronal culture
rat synaptosomes

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1007/BF00974574

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title = "On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes",

abstract = "The uptake of radioactive γ-aminobutyric acid (GABA) and d-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [3H]GABA and for d-[3H]aspartate with an increasing Vmax during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake of d-aspartate. We found no non-saturable uptake of d-aspartate.",

keywords = "GABA, SKF 89976-A, d-aspartate, glutamate, primary neuronal culture, rat synaptosomes",

author = "Lena Lewin and Mattsson, {Mats Olof} and Rassin, {David K.} and {\AA}ke Sellstr{\"o}m",

year = "1992",

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doi = "10.1007/BF00974574",

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AU - Lewin, Lena

AU - Mattsson, Mats Olof

AU - Rassin, David K.

AU - Sellström, Åke

PY - 1992/4

Y1 - 1992/4

N2 - The uptake of radioactive γ-aminobutyric acid (GABA) and d-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [3H]GABA and for d-[3H]aspartate with an increasing Vmax during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake of d-aspartate. We found no non-saturable uptake of d-aspartate.

AB - The uptake of radioactive γ-aminobutyric acid (GABA) and d-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [3H]GABA and for d-[3H]aspartate with an increasing Vmax during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake of d-aspartate. We found no non-saturable uptake of d-aspartate.

KW - GABA

KW - SKF 89976-A

KW - d-aspartate

KW - glutamate

KW - primary neuronal culture

KW - rat synaptosomes

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On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes

Abstract

Keywords

ASJC Scopus subject areas

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