Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity

Yan Liu; Zhikai Zhang; Yongquan Jiang; Lihong Zhang; Vsevolod L. Popov; Jianzhi Zhang; David H. Walker; Xue jie Yu

doi:10.1016/j.micinf.2010.10.021

Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity

Yan Liu, Zhikai Zhang, Yongquan Jiang, Lihong Zhang, Vsevolod L. Popov, Jianzhi Zhang, David H. Walker, Xue jie Yu

Pathology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.

Original language	English (US)
Pages (from-to)	232-238
Number of pages	7
Journal	Microbes and Infection
Volume	13
Issue number	3
DOIs	https://doi.org/10.1016/j.micinf.2010.10.021
State	Published - Mar 2011

Keywords

Ehrlichia
Ehrlichiosis
Mitochondria

ASJC Scopus subject areas

Microbiology
Immunology
Infectious Diseases

Access to Document

10.1016/j.micinf.2010.10.021

Cite this

@article{e6677ca6d3114ce7a1ae64d6456708e1,

title = "Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity",

abstract = "Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.",

keywords = "Ehrlichia, Ehrlichiosis, Mitochondria",

author = "Yan Liu and Zhikai Zhang and Yongquan Jiang and Lihong Zhang and Popov, {Vsevolod L.} and Jianzhi Zhang and Walker, {David H.} and Yu, {Xue jie}",

note = "Funding Information: This study was supported in part by a grant from National Institute of Allergy and Infectious Diseases ( AI31431 ). The authors are grateful to Professor Thomas Albrecht (Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas) for technical assistance with confocal microscopic imaging.",

year = "2011",

month = mar,

doi = "10.1016/j.micinf.2010.10.021",

language = "English (US)",

volume = "13",

pages = "232--238",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier Masson s.r.l.",

number = "3",

}

TY - JOUR

T1 - Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity

AU - Liu, Yan

AU - Zhang, Zhikai

AU - Jiang, Yongquan

AU - Zhang, Lihong

AU - Popov, Vsevolod L.

AU - Zhang, Jianzhi

AU - Walker, David H.

AU - Yu, Xue jie

N1 - Funding Information: This study was supported in part by a grant from National Institute of Allergy and Infectious Diseases ( AI31431 ). The authors are grateful to Professor Thomas Albrecht (Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas) for technical assistance with confocal microscopic imaging.

PY - 2011/3

Y1 - 2011/3

N2 - Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.

AB - Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.

KW - Ehrlichia

KW - Ehrlichiosis

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=78951491174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78951491174&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2010.10.021

DO - 10.1016/j.micinf.2010.10.021

M3 - Article

C2 - 21070861

AN - SCOPUS:78951491174

SN - 1286-4579

VL - 13

SP - 232

EP - 238

JO - Microbes and Infection

JF - Microbes and Infection

IS - 3

ER -

Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this