TY - JOUR
T1 - Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype
AU - Deubel, Vincent
AU - Kinney, Richard M.
AU - Trent, Dennis W.
N1 - Funding Information:
We thank Dr. Vance Vorndam for providing the synthesized oligodeoxynucleotides, Dr. Barbara Johnson and Dr. Gwong Jen Chang for helpful suggestions during the course of this study, and Dr. Thomas Monath for his advice in the preparation of this manuscript. V.D. is a microbiologist at the Pasteur Institute, Laboratory of Molecular Virology, Paris (France). This research was supported in part by the Direction des Recherches Etudes et Techniques 85 34 820 00 470 75 01 (Paris) and by Fellowship 1 F05 TWO 3596 from the International Research and Awards Branch, National Institutes of Health, Bethesda, Maryland.
PY - 1986/12
Y1 - 1986/12
N2 - The nucleotide sequence of the 5′-terminal 2469 bases of dengue 2 (Jamaica genotype) virus has been determined and the encoded proteins compared with those of yellow fever and West Nile viruses, which belong to different flavivirus serogroups. The cDNA clone which was sequenced contains a 5′-noncoding region of 96 nucleotides followed by a single open reading frame coding for the structural proteins 5′-C-prM(M)-E-3′ and the beginning of the NS1 nonstructural protein. The amino acid sequence homology between the structural polyprotein precursor of dengue 2 virus and those of yellow fever and West Nile viruses is 36.5 and 42%, respectively. The dengue virus structural proteins are similar in size and composition to those of the other flaviviruses. The basic capsid protein and the membrane and envelope proteins have hydrophobic regions at their C termini. The dengue 2 capsid C, membrane M, and envelope E proteins share 13, 36, and 43% homology, respectively, with the cognate proteins of yellow fever virus, and 33, 32, and 47% homology with the cognate proteins of West Nile virus. All 6 cysteine residues in the dengue 2 premembrane protein and all 12 cysteine residues in the dengue 2 envelope protein are conserved in the cognate proteins of yellow fever and West Nile viruses.
AB - The nucleotide sequence of the 5′-terminal 2469 bases of dengue 2 (Jamaica genotype) virus has been determined and the encoded proteins compared with those of yellow fever and West Nile viruses, which belong to different flavivirus serogroups. The cDNA clone which was sequenced contains a 5′-noncoding region of 96 nucleotides followed by a single open reading frame coding for the structural proteins 5′-C-prM(M)-E-3′ and the beginning of the NS1 nonstructural protein. The amino acid sequence homology between the structural polyprotein precursor of dengue 2 virus and those of yellow fever and West Nile viruses is 36.5 and 42%, respectively. The dengue virus structural proteins are similar in size and composition to those of the other flaviviruses. The basic capsid protein and the membrane and envelope proteins have hydrophobic regions at their C termini. The dengue 2 capsid C, membrane M, and envelope E proteins share 13, 36, and 43% homology, respectively, with the cognate proteins of yellow fever virus, and 33, 32, and 47% homology with the cognate proteins of West Nile virus. All 6 cysteine residues in the dengue 2 premembrane protein and all 12 cysteine residues in the dengue 2 envelope protein are conserved in the cognate proteins of yellow fever and West Nile viruses.
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U2 - 10.1016/0042-6822(86)90200-X
DO - 10.1016/0042-6822(86)90200-X
M3 - Article
C2 - 3024394
AN - SCOPUS:0022891635
SN - 0042-6822
VL - 155
SP - 365
EP - 377
JO - Virology
JF - Virology
IS - 2
ER -