@article{40776c2a00874453a827b82908407f64,
title = "Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing",
abstract = "We present a genome-wide approach to map DNA double-strand breaks (DSBs) at nucleotide resolution by a method we termed BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing). We validated and tested BLESS using human and mouse cells and different DSBs-inducing agents and sequencing platforms. BLESS was able to detect telomere ends, Sce endonuclease-induced DSBs and complex genome-wide DSB landscapes. As a proof of principle, we characterized the genomic landscape of sensitivity to replication stress in human cells, and we identified >2,000 nonuniformly distributed aphidicolin-sensitive regions (ASRs) overrepresented in genes and enriched in satellite repeats. ASRs were also enriched in regions rearranged in human cancers, with many cancer-associated genes exhibiting high sensitivity to replication stress. Our method is suitable for genome-wide mapping of DSBs in various cells and experimental conditions, with a specificity and resolution unachievable by current techniques.",
author = "Nicola Crosetto and Abhishek Mitra and Silva, {Maria Joao} and Magda Bienko and Norbert Dojer and Qi Wang and Elif Karaca and Roberto Chiarle and Magdalena Skrzypczak and Krzysztof Ginalski and Philippe Pasero and Maga Rowicka and Ivan Dikic",
note = "Funding Information: We acknowledge Y. Shiloh (Tel Aviv University) and A.J. Pierce (University of Kentucky) for kindly providing U2OS_DRH-1 cells and I-Sce plasmids. We are grateful to T. W{\l}odarski, A.R. Lehmann, G. Fudenberg and A. Kudlicki for insightful discussions, critical reading of the manuscript and help with data analysis. This work was supported by grants from Deutsche Forschungsgemeinschaft, the Cluster of Excellence “Macromolecular Complexes” of the Goethe University Frankfurt (EXC115), the LOEWE-funded Oncogenic Signaling Frankfurt network, the LOEWE Gene and Cell Therapy Center and the European Research Council (ERC) under the European Union{\textquoteright}s Seventh Framework Programme (FP7/2007-2013) and ERC grant agreement 250241-LineUb to I.D.; from the Associazione Italiana per la Ricerca sul cancro (AIRC) and the International Association for Cancer Research (AICR) and grant FP7 ERC-2009-StG (proposal 242965–“Lunely”) to R.C.; from the Foundation for Polish Science (TEAM), Polish National Science Centre (2011/02/A/NZ2/00014) and European Regional Development Fund under the Innovative Economy Programme (POIG.02.02.00-14-024/08-00) to K.G.; from Ligue contre le Cancer ({\'e}quipe labellis{\'e}e), Agence Nationale de la Recherche (RepliCare) and Institut National du Cancer to P.P.; and by grant UL1TR000071 ITS “Novel Methods” from the National Center for Research Resources, US National Institutes of Health, to M.R. M.B. is a recipient of a Human Frontier Science Program Long-Term Fellowship.",
year = "2013",
month = apr,
doi = "10.1038/nmeth.2408",
language = "English (US)",
volume = "10",
pages = "361--365",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "4",
}