TY - JOUR
T1 - Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells
AU - Okorodudu, Anthony O.
AU - Lambert, W. Clark
AU - Lambert, Muriel W.
N1 - Funding Information:
This work was supported in part by Basil O'Connor Grant #5-287 from the March of Dimes Birth Defects Foundation, the Dermatology Foundation and the Fourrlation of the UIW.
PY - 1982/9/30
Y1 - 1982/9/30
N2 - Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.
AB - Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.
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U2 - 10.1016/0006-291X(82)90867-1
DO - 10.1016/0006-291X(82)90867-1
M3 - Article
C2 - 6216888
AN - SCOPUS:0020457568
SN - 0006-291X
VL - 108
SP - 576
EP - 584
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -