Noninvasive assessment of ocular tissue using confocal Raman spectroscopy

James P. Wicksted, Noel J.C. Bauer, Roel J. Erckens, Franciscus H.M. Jongsma, Eefke E. Smit-Kepel, Massoud Motamedi, Wayne F. March

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


We have developed a confocal Raman spectroscopy system in order to noninvasively characterize ocular tissue with both an in vitro and in vivo capability. This systems consists of a long working distance optical probe, which focuses the incident laser light onto the tissue and collects the backscattered Raman signal, a single grating spectrometer with CCD camera, and an optical fiber which couples the optical probe to the spectrometer. Essential to the confocal design is that the sample volume is limited, preventing detection of interference signals and fluorescence from adjacent ocular tissues. This sample volume is adjustable by changing the diameter of the collection fiber which acts as the pinhole in the system. Potential applications of this technique such as assessing corneal hydration and quantifying pharmacokinetic drug transport across the cornea will be discussed.

Original languageEnglish (US)
Pages (from-to)112-122
Number of pages11
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 Biomedical Applications of Raman Spectroscopy - San Jose, Ca, USA
Duration: Jan 25 1999Jan 26 1999

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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