NMR Studies on an Oligodeoxynucleotide Containing 2-Aminopurine opposite Adenine

A heteroduplex containing the mismatch 2-aminopurine (AP)-adenine has been synthesized and studied by proton NMR. The mismatch was incorporated into the seuquence d[CGG(AP)GGC].d-(GCCACCG). One-dimensional nuclear Overhauser effect measurements in H₂0 and two-dimensional nuclear Overhauser effect spectra in D₂0 show AP-A base pairs in a wobble structure in which both bases are in the anti conformation. The adenine is stacked well in the helix, but the helix twist between the adenine and neighboring cytosine in the 3^/direction is unusually small. As a result, the aminopurine on the opposite strand is somewhat pushed out of the helix. From the measurements of the imino proton line widths, the two adjacent G-C base pairs are not found to be significantly destabilized by the presence of the purine-purine wobble pair.