TY - JOUR
T1 - Neonatal lead toxicity and in vitro lipid peroxidation of rat brain
AU - Gelman, Benjamin B.
AU - Michaelson, I. Arthur
N1 - Funding Information:
We thank Dr. Klaus L. Stemmer for evaluation of brain tissue pathology. Supported by National Institute of Environmental Health Sciences grant ES-00127 and a Chemical Industry Institute of Toxicology predoctoral fellowship (to B.B.G.). Requests for reprints should be sent to B. B. Gelman, Department of Biological Chemistry, University of Cincinnati College of Medicine, 3223 Eden Avenue, Cincinnati, Ohio 45267.
PY - 1979/7
Y1 - 1979/7
N2 - Neonatal rats were given aqueous lead acetate intragastrically from d 2-20 of life at doses of 0, 25, 75, and 225 mg Pb/kg·d. Blood Pb concentrations on d 21 were (mean ± SE) 27 ± 4 (control), 150 ± 26, 263 ± 63, and 518 ± 97 μg/100 ml, respectively. Growth was significantly depressed only in animals given the highest dose of Pb (225 mg/kg·d). Hematocrits were significantly decreased by d21 at all doses of Pb. Malondialdehyde (MDA) formation in 750 X g (10 min) brain supernatants induced spontaneously by aerobic incubation at 37°C was not altered by Pb on d 7 and 14, but a slight decrease was observed on d 21. The extent of MDA formation induced by enzymatically generated superoxide anion was not altered by Pb toxicity during the first 21 d of life. Addition of Pb to 750 X g (10 min) brain supernatants in vitro significantly decreased MDA formation at Pb concentrations of 10-5 M and higher. These results show that the central nervous system toxicity of Pb in neonatal rats is not associated with accelerated in vitro Iipid peroxidation of brain tissue.
AB - Neonatal rats were given aqueous lead acetate intragastrically from d 2-20 of life at doses of 0, 25, 75, and 225 mg Pb/kg·d. Blood Pb concentrations on d 21 were (mean ± SE) 27 ± 4 (control), 150 ± 26, 263 ± 63, and 518 ± 97 μg/100 ml, respectively. Growth was significantly depressed only in animals given the highest dose of Pb (225 mg/kg·d). Hematocrits were significantly decreased by d21 at all doses of Pb. Malondialdehyde (MDA) formation in 750 X g (10 min) brain supernatants induced spontaneously by aerobic incubation at 37°C was not altered by Pb on d 7 and 14, but a slight decrease was observed on d 21. The extent of MDA formation induced by enzymatically generated superoxide anion was not altered by Pb toxicity during the first 21 d of life. Addition of Pb to 750 X g (10 min) brain supernatants in vitro significantly decreased MDA formation at Pb concentrations of 10-5 M and higher. These results show that the central nervous system toxicity of Pb in neonatal rats is not associated with accelerated in vitro Iipid peroxidation of brain tissue.
UR - http://www.scopus.com/inward/record.url?scp=0018750589&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018750589&partnerID=8YFLogxK
U2 - 10.1080/15287397909529779
DO - 10.1080/15287397909529779
M3 - Article
C2 - 490679
AN - SCOPUS:0018750589
SN - 0098-4108
VL - 5
SP - 671
EP - 682
JO - Journal of Toxicology and Environmental Health
JF - Journal of Toxicology and Environmental Health
IS - 4
ER -