N-acetyl-β-hexosaminidase: Role in the degradation of glycosaminoglycans

Jerry N. Thompson; Allen C. Stoolmiller; Reuben Matalon; Albert Dorfman

doi:10.1126/science.181.4102.866

N-acetyl-β-hexosaminidase: Role in the degradation of glycosaminoglycans

Jerry N. Thompson, Allen C. Stoolmiller, Reuben Matalon, Albert Dorfman

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Extracts of cultured normal human skin fibroblasts released radioactivity from a ¹⁴C-labeled heptasaccharide prepared by addition of [ ¹⁴C]N-acetylgalactosamine to the nonreducing terminus of a hexasaccharide derived from chondroitin 4-sulfate whereas fibroblast extracts from patients with Tay-Sachs and Sandhoff-Jatzkewitz diseases did not. The results suggest that N-acetyl-β-hexosaminidase A is responsible for degradation of the oligosaccharide substrate.

Original language	English (US)
Pages (from-to)	866-867
Number of pages	2
Journal	Science
Volume	181
Issue number	4102
DOIs	https://doi.org/10.1126/science.181.4102.866
State	Published - 1973
Externally published	Yes

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title = "N-acetyl-β-hexosaminidase: Role in the degradation of glycosaminoglycans",

abstract = "Extracts of cultured normal human skin fibroblasts released radioactivity from a 14C-labeled heptasaccharide prepared by addition of [ 14C]N-acetylgalactosamine to the nonreducing terminus of a hexasaccharide derived from chondroitin 4-sulfate whereas fibroblast extracts from patients with Tay-Sachs and Sandhoff-Jatzkewitz diseases did not. The results suggest that N-acetyl-β-hexosaminidase A is responsible for degradation of the oligosaccharide substrate.",

author = "Thompson, {Jerry N.} and Stoolmiller, {Allen C.} and Reuben Matalon and Albert Dorfman",

year = "1973",

doi = "10.1126/science.181.4102.866",

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T2 - Role in the degradation of glycosaminoglycans

AU - Thompson, Jerry N.

AU - Stoolmiller, Allen C.

AU - Matalon, Reuben

AU - Dorfman, Albert

PY - 1973

Y1 - 1973

N2 - Extracts of cultured normal human skin fibroblasts released radioactivity from a 14C-labeled heptasaccharide prepared by addition of [ 14C]N-acetylgalactosamine to the nonreducing terminus of a hexasaccharide derived from chondroitin 4-sulfate whereas fibroblast extracts from patients with Tay-Sachs and Sandhoff-Jatzkewitz diseases did not. The results suggest that N-acetyl-β-hexosaminidase A is responsible for degradation of the oligosaccharide substrate.

AB - Extracts of cultured normal human skin fibroblasts released radioactivity from a 14C-labeled heptasaccharide prepared by addition of [ 14C]N-acetylgalactosamine to the nonreducing terminus of a hexasaccharide derived from chondroitin 4-sulfate whereas fibroblast extracts from patients with Tay-Sachs and Sandhoff-Jatzkewitz diseases did not. The results suggest that N-acetyl-β-hexosaminidase A is responsible for degradation of the oligosaccharide substrate.

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N-acetyl-β-hexosaminidase: Role in the degradation of glycosaminoglycans

Abstract

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