Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids: Extinction of α-fetoprotein gene

Wesley K. Church; John Papaconstantinou; Sau Wah Kwan; Anne Poliard; Claude Szpirer; Josianne Szpirer

doi:10.1007/BF01534859

Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids: Extinction of α-fetoprotein gene

Wesley K. Church, John Papaconstantinou, Sau Wah Kwan, Anne Poliard, Claude Szpirer, Josianne Szpirer

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Somatic cell hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse AFP gene expression. Analysis of HindIII digests clearly shows that both mouse and rat AFP genes are present in the hybrids. DNA-RNA hybridization data show that AFP mRNA is virtually absent in the somatic cell hybrids. Our studies indicate, therefore, that extinction of mouse AFP gene expression in somatic cell hybrids (BJ01, BJ50, and BJ140) occurs in the presence of the rat genes and that the mechanism of extinction involves processes that result in the loss of AFP mRNA. We propose that the mechanism may involve either the inhibition of transcription or an inhibition of the processing of the mRNA transcript.

Original language	English (US)
Pages (from-to)	541-545
Number of pages	5
Journal	Somatic Cell and Molecular Genetics
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/BF01534859
State	Published - Sep 1984

ASJC Scopus subject areas

Genetics
Cell Biology

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title = "Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids: Extinction of α-fetoprotein gene",

abstract = "Somatic cell hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse AFP gene expression. Analysis of HindIII digests clearly shows that both mouse and rat AFP genes are present in the hybrids. DNA-RNA hybridization data show that AFP mRNA is virtually absent in the somatic cell hybrids. Our studies indicate, therefore, that extinction of mouse AFP gene expression in somatic cell hybrids (BJ01, BJ50, and BJ140) occurs in the presence of the rat genes and that the mechanism of extinction involves processes that result in the loss of AFP mRNA. We propose that the mechanism may involve either the inhibition of transcription or an inhibition of the processing of the mRNA transcript.",

author = "Church, {Wesley K.} and John Papaconstantinou and Kwan, {Sau Wah} and Anne Poliard and Claude Szpirer and Josianne Szpirer",

year = "1984",

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T1 - Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids

T2 - Extinction of α-fetoprotein gene

AU - Church, Wesley K.

AU - Papaconstantinou, John

AU - Kwan, Sau Wah

AU - Poliard, Anne

AU - Szpirer, Claude

AU - Szpirer, Josianne

PY - 1984/9

Y1 - 1984/9

N2 - Somatic cell hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse AFP gene expression. Analysis of HindIII digests clearly shows that both mouse and rat AFP genes are present in the hybrids. DNA-RNA hybridization data show that AFP mRNA is virtually absent in the somatic cell hybrids. Our studies indicate, therefore, that extinction of mouse AFP gene expression in somatic cell hybrids (BJ01, BJ50, and BJ140) occurs in the presence of the rat genes and that the mechanism of extinction involves processes that result in the loss of AFP mRNA. We propose that the mechanism may involve either the inhibition of transcription or an inhibition of the processing of the mRNA transcript.

AB - Somatic cell hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse AFP gene expression. Analysis of HindIII digests clearly shows that both mouse and rat AFP genes are present in the hybrids. DNA-RNA hybridization data show that AFP mRNA is virtually absent in the somatic cell hybrids. Our studies indicate, therefore, that extinction of mouse AFP gene expression in somatic cell hybrids (BJ01, BJ50, and BJ140) occurs in the presence of the rat genes and that the mechanism of extinction involves processes that result in the loss of AFP mRNA. We propose that the mechanism may involve either the inhibition of transcription or an inhibition of the processing of the mRNA transcript.

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Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids: Extinction of α-fetoprotein gene

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