Molecular diagnosis of bovine tuberculosis in bovine and human samples: Implications for zoonosis

Md Masudur Rahman; Monira Noor; Kazi Mehetazul Islam; Md Bashir Uddin; Ferdaus Mohd Altaf Hossain; Mohammad Ali Zinnah; Mohammad Al Mamun; Mohammad Rafiqul Islam; Seong Kug Eo; Hossam M. Ashour

doi:10.2217/fmb.14.139

Molecular diagnosis of bovine tuberculosis in bovine and human samples: Implications for zoonosis

Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo, Hossam M. Ashour

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

Original language	English (US)
Pages (from-to)	527-535
Number of pages	9
Journal	Future Microbiology
Volume	10
Issue number	4
DOIs	https://doi.org/10.2217/fmb.14.139
State	Published - Apr 1 2015
Externally published	Yes

Keywords

bovine tuberculosis
molecular diagnosis
zoonotic transmission

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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10.2217/fmb.14.139

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title = "Molecular diagnosis of bovine tuberculosis in bovine and human samples: Implications for zoonosis",

abstract = "Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.",

keywords = "bovine tuberculosis, molecular diagnosis, zoonotic transmission",

author = "Rahman, {Md Masudur} and Monira Noor and Islam, {Kazi Mehetazul} and Uddin, {Md Bashir} and Hossain, {Ferdaus Mohd Altaf} and Zinnah, {Mohammad Ali} and Mamun, {Mohammad Al} and Islam, {Mohammad Rafiqul} and Eo, {Seong Kug} and Ashour, {Hossam M.}",

note = "Publisher Copyright: {\textcopyright} 2015 Future Medicine Ltd.",

year = "2015",

month = apr,

day = "1",

doi = "10.2217/fmb.14.139",

language = "English (US)",

volume = "10",

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journal = "Future Microbiology",

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publisher = "Future Medicine Ltd.",

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TY - JOUR

T1 - Molecular diagnosis of bovine tuberculosis in bovine and human samples

T2 - Implications for zoonosis

AU - Rahman, Md Masudur

AU - Noor, Monira

AU - Islam, Kazi Mehetazul

AU - Uddin, Md Bashir

AU - Hossain, Ferdaus Mohd Altaf

AU - Zinnah, Mohammad Ali

AU - Mamun, Mohammad Al

AU - Islam, Mohammad Rafiqul

AU - Eo, Seong Kug

AU - Ashour, Hossam M.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

AB - Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

KW - bovine tuberculosis

KW - molecular diagnosis

KW - zoonotic transmission

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Molecular diagnosis of bovine tuberculosis in bovine and human samples: Implications for zoonosis

Abstract

Keywords

ASJC Scopus subject areas

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