Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae

Louise Prakash; Dennis Dumais; Renata Polakowska; Giuditta Perozzi; Satya Prakash

doi:10.1016/0378-1119(85)90294-X

Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae

Louise Prakash, Dennis Dumais, Renata Polakowska, Giuditta Perozzi, Satya Prakash

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We have cloned the RAD10 gene of Saccharomyces cerevisiae and physically mapped it to a 1.0-kb DNA fragment. Strains containing disruptions of the RAD10 gene were found to show enhanced UV sensitivity compared with the previously characterized rad10-1 or rad10-2 mutants. The UV sensitivity of the disruption mutant is comparable to the highly UV sensitive rad1-19, rad2-Δ, and rad3-2 mutants.

Original language	English (US)
Pages (from-to)	55-61
Number of pages	7
Journal	Gene
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(85)90294-X
State	Published - 1985
Externally published	Yes

Keywords

DNA repair
M13 phage vector
Recombinant DNA
hypersensitivity
pyrimidine dimers
radiation sensitivity
yeast

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(85)90294-X

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title = "Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae",

abstract = "We have cloned the RAD10 gene of Saccharomyces cerevisiae and physically mapped it to a 1.0-kb DNA fragment. Strains containing disruptions of the RAD10 gene were found to show enhanced UV sensitivity compared with the previously characterized rad10-1 or rad10-2 mutants. The UV sensitivity of the disruption mutant is comparable to the highly UV sensitive rad1-19, rad2-Δ, and rad3-2 mutants.",

keywords = "DNA repair, M13 phage vector, Recombinant DNA, hypersensitivity, pyrimidine dimers, radiation sensitivity, yeast",

author = "Louise Prakash and Dennis Dumais and Renata Polakowska and Giuditta Perozzi and Satya Prakash",

note = "Funding Information: This investigationw as supported by grants CA35035t o S.P. and GM19261t o L.P. from NIH, and contract no. DE-ACO2-74EVO3490w ith the U.S. Departmento f Energy at the University of RochesterD epartmento f Radiation Biology and Biophysics and has been assignedr eport No. DOE/EV/03490-2429.",

year = "1985",

doi = "10.1016/0378-1119(85)90294-X",

language = "English (US)",

volume = "34",

pages = "55--61",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

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}

TY - JOUR

T1 - Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae

AU - Prakash, Louise

AU - Dumais, Dennis

AU - Polakowska, Renata

AU - Perozzi, Giuditta

AU - Prakash, Satya

N1 - Funding Information: This investigationw as supported by grants CA35035t o S.P. and GM19261t o L.P. from NIH, and contract no. DE-ACO2-74EVO3490w ith the U.S. Departmento f Energy at the University of RochesterD epartmento f Radiation Biology and Biophysics and has been assignedr eport No. DOE/EV/03490-2429.

PY - 1985

Y1 - 1985

N2 - We have cloned the RAD10 gene of Saccharomyces cerevisiae and physically mapped it to a 1.0-kb DNA fragment. Strains containing disruptions of the RAD10 gene were found to show enhanced UV sensitivity compared with the previously characterized rad10-1 or rad10-2 mutants. The UV sensitivity of the disruption mutant is comparable to the highly UV sensitive rad1-19, rad2-Δ, and rad3-2 mutants.

AB - We have cloned the RAD10 gene of Saccharomyces cerevisiae and physically mapped it to a 1.0-kb DNA fragment. Strains containing disruptions of the RAD10 gene were found to show enhanced UV sensitivity compared with the previously characterized rad10-1 or rad10-2 mutants. The UV sensitivity of the disruption mutant is comparable to the highly UV sensitive rad1-19, rad2-Δ, and rad3-2 mutants.

KW - DNA repair

KW - M13 phage vector

KW - Recombinant DNA

KW - hypersensitivity

KW - pyrimidine dimers

KW - radiation sensitivity

KW - yeast

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DO - 10.1016/0378-1119(85)90294-X

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VL - 34

SP - 55

EP - 61

JO - Gene

JF - Gene

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ER -

Molecular cloning of the RAD10 gene of Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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