Molecular cloning and expression of a human B-cell growth factor gene in Escherichia coli

Surendra Sharma, Shashi Mehta, John Morgan, Abby Maizel

Research output: Contribution to journalArticlepeer-review

Abstract

A human B-cell growth factor (BCGF) (12 kilodaltons) supports the clonal proliferation of B lymphocytes. A clone was isolated that contained the proper structural sequence to encode biologically active, 12-kilodalton BCGF in Escherichia coli and to hybridize to a specific messenger RNA, identified by in vitro translation in Xenopus laevis oocytes. A relatively hydrophobic region of 18 amino acids was found at the amino terminal of the 124-amino acid-long polypeptide. The carboxly terminal is composed of at least 32 amino adds that are derived from nucleotide sequences bearing significant homology to the Alu repeat family.

Original languageEnglish (US)
Pages (from-to)1489-1492
Number of pages4
JournalScience
Volume235
Issue number4795
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • General

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