TY - JOUR
T1 - Molecular characterization of M1146, an American isolate of Ljungan virus (LV) reveals the presence of a new LV genotype
AU - Johansson, E. Susanne
AU - Niklasson, Bo
AU - Tesh, Robert B.
AU - Shafren, Darren R.
AU - Travassos da Rosa, Amelia P.A.
AU - Lindberg, A. Michael
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Ljungan virus (LV) is a suspected human pathogen recently isolated from bank voles in Sweden. This study describes the genetic characterization of a virus, M1146, which was isolated in 1962 from another vole species (Microtus montanus), trapped in Oregon, USA. Based on antigenic properties, M1146 was postulated previously as a putative member of the family Picornaviridae. The near complete genomic sequence verifies that M1146 is a member of the Picornaviridae, most closely related to LVs isolated in Sweden. The strain M1146 possesses typical LV genomic organization, including a cluster of two 2A homologues. There are significant differences throughout the capsid protein region, while the non-structural region of M1146 is closely related to the Swedish LV genomes. Genetic and phylogenetic analyses show that M1146 represents a new genotype within the distinct LV cluster. Isolation of LV from both Swedish and American voles trapped over a period of 30 years suggests a continuous worldwide presence.
AB - Ljungan virus (LV) is a suspected human pathogen recently isolated from bank voles in Sweden. This study describes the genetic characterization of a virus, M1146, which was isolated in 1962 from another vole species (Microtus montanus), trapped in Oregon, USA. Based on antigenic properties, M1146 was postulated previously as a putative member of the family Picornaviridae. The near complete genomic sequence verifies that M1146 is a member of the Picornaviridae, most closely related to LVs isolated in Sweden. The strain M1146 possesses typical LV genomic organization, including a cluster of two 2A homologues. There are significant differences throughout the capsid protein region, while the non-structural region of M1146 is closely related to the Swedish LV genomes. Genetic and phylogenetic analyses show that M1146 represents a new genotype within the distinct LV cluster. Isolation of LV from both Swedish and American voles trapped over a period of 30 years suggests a continuous worldwide presence.
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U2 - 10.1099/vir.0.18792-0
DO - 10.1099/vir.0.18792-0
M3 - Short survey
C2 - 12655084
AN - SCOPUS:0037385514
SN - 0022-1317
VL - 84
SP - 837
EP - 844
JO - Journal of General Virology
JF - Journal of General Virology
IS - 4
ER -